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A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile
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SYSNO ASEP 0458757 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A previously undescribed jasmonate compound in flowering Arabidopsis thaliana - The identification of cis-(+)-OPDA-Ile Tvůrce(i) Floková, K. (CZ)
Feussner, K. (DE)
Herrfurth, C. (DE)
Miersch, O. (CZ)
Mik, V. (CZ)
Tarkowská, Danuše (UEB-Q) RID, ORCID
Strnad, Miroslav (UEB-Q) RID, ORCID
Feussner, I. (DE)
Wasternack, Claus (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAIZdroj.dok. Phytochemistry. - : Elsevier - ISSN 0031-9422
Roč. 122, FEB (2016), s. 230-237Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis thaliana (Brassicaceae) ; Jasmonates ; Cis-(+)-12-oxo-phytodienoyl-L-isoleucine Vědní obor RIV EB - Genetika a molekulární biologie CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LK21306 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000369460200025 DOI 10.1016/j.phytochem.2015.11.012 Anotace Jasmonates (JAs) are plant hormones that integrate external stress stimuli with physiological responses. (+)-7-iso-JA-L-Ile is the natural JA ligand of COI1, a component of a known JA receptor. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-(+)-OPDA) has been reported to act independently of COI1 as an essential signal in several stress-induced and developmental processes. Wound induced increases in the endogenous levels of JA/JA-Ile are accompanied by two to tenfold increases in the concentration of OPDA, but its means of perception and metabolism are unknown. To screen for putative OPDA metabolites, vegetative tissues of flowering Arabidopsis thaliana were extracted with 25% aqueous methanol (v/v), purified by single-step reversed-phase polymer-based solid-phase extraction, and analyzed by high throughput mass spectrometry. This enabled the detection and quantitation of a low abundant OPDA analog of the biologically active (+)-7-iso-JA-L-Ile in plant tissue samples. Levels of the newly identified compound and the related phytohormones JA, JA-Ile and cis-(+)-OPDA were monitored in wounded leaves of flowering Arabidopsis lines (Col-0 and Ws) and compared to the levels observed in Arabidopsis mutants deficient in the biosynthesis of JA (dde2-2, opr3) and JA-Ile (jarl). The observed cis-(+)-OPDA-Ile levels varied widely, raising,questions concerning its role in Arabidopsis stress responses. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
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