Počet záznamů: 1
Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents
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SYSNO ASEP 0457384 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents Tvůrce(i) Pasdar, E.A. (AU)
Smits, M. (AU)
Stapelberg, M. (AU)
Bajziková, Martina (BTO-N) RID
Stantic, M. (AU)
Goodwin, J. (AU)
Yan, B. (AU)
Štursa, J. (CZ)
Kovářová, Jaromíra (BTO-N) RID
Sachaphibulkij, K. (AU)
Bezawork-Geleta, A. (AU)
Sobol, Margaryta (UMG-J) RID
Philimonenko, Anatoly (UMG-J)
Tomasetti, M. (IT)
Zobalová, Renata (BTO-N) RID
Hozák, Pavel (UMG-J) RID, ORCID
Dong, L.F. (AU)
Neužil, Jiří (BTO-N) RIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 10, č. 5 (2015), e0119549Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MALIGNANT PLEURAL MESOTHELIOMA ; EMBRYONIC STEM-CELLS ; ALPHA-TOCOPHERYL SUCCINATE Vědní obor RIV EB - Genetika a molekulární biologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000353887100002 DOI 10.1371/journal.pone.0119549 Anotace Malignant mesothelioma (MM) is an aggressive type of tumour causing high mortality. One reason for this paradigm may be the existence of a subpopulation of tumour-initiating cells (TICs) that endow MM with drug resistance and recurrence. The objective of this study was to identify and characterise a TIC subpopulation in MM cells, using spheroid cultures, mesospheres, as a model of MM TICs. Mesospheres, typified by the stemness markers CD24, ABCG2 and OCT4, initiated tumours in immunodeficient mice more efficiently than adherent cells. CD24 knock-down cells lost the sphere-forming capacity and featured lower tumorigenicity. Upon serial transplantation, mesospheres were gradually more efficiently tumrigenic with increased level of stem cell markers. We also show that mesospheres feature mitochondrial and metabolic properties similar to those of normal and cancer stem cells. Finally, we show that mesothelioma-initiating cells are highly susceptible to mitochondrially targeted vitamin E succinate. This study documents that mesospheres can be used as a plausible model of mesothelioma-initiating cells and that they can be utilised in the search for efficient agents against MM. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2016
Počet záznamů: 1