Počet záznamů: 1
G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques
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SYSNO ASEP 0456709 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques Tvůrce(i) Vidláková, Pavlína (BFU-R)
Pivoňková, Hana (BFU-R) ORCID
Kejnovská, Iva (BFU-R) RID, ORCID
Trnková, L. (CZ)
Vorlíčková, Michaela (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCID
Havran, Luděk (BFU-R) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 407, č. 19 (2015), s. 5817-5826Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Oligonucleotides ; Electrochemical methods ; G-quadruplex Vědní obor RIV BO - Biofyzika CEP GAP206/12/2378 GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000358136900025 DOI 10.1007/s00216-015-8768-1 Anotace Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2016
Počet záznamů: 1