Počet záznamů: 1

Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp

SYSNO ASEP	0455568
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp
Tvůrce(i)	Grulich, Michal (MBU-M)_RID Brezovský, J. (CZ) Štěpánek, Václav (MBU-M)_{RID, ORCID} Palyzová, Andrea (MBU-M)_{RID, ORCID} Kyslíková, Eva (MBU-M) Kyslík, Pavel (MBU-M)_{RID, ORCID}
Zdroj.dok.	Journal of Molecular Catalysis B-Enzymatic. - : Elsevier - ISSN 1381-1177 Roč. 122, DEC 2015 (2015), s. 240-247
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Penicillin G acylase ; Enantioselectivity ; Homologous model
Vědní obor RIV	CE - Biochemie
CEP	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000366078800030
DOI	10.1016/j.molcatb.2015.09.008
Anotace	Penicillin G acylases (PGAs) are enantioselective enzymes catalyzing a hydrolysis of stable amide bond in a broad spectrum of substrates. Among them, derivatives of alpha- and beta-amino acids represent a class of compounds with high application potential. PGA(Ec) from Escherichia coil and PGA(A) from Achromobacter sp. CCM 4824 were used to catalyze enantioselective hydrolyses of seven selected N-phenylacetylated alpha/beta-amino acid racemates. The PGA(A) showed higher stereoselectivity for enantiomers of N-PhA-beta-homoleucine, N-PhAc-alpha-tert-leucine and N-PhAc-beta-leucine. To study the mechanism of enantiodiscrimination on molecular level, we have constructed a homology model of PGA(A) that was used in molecular docking experiments with the same substrates. In-silico experiments successfully reproduced the data from experimental enzymatic resolutions confirming validity of employed modeling protocol. We employed this protocol to evaluate enantiopreference of PGA(A) towards seven new substrates with application potential. For five of them, high enantioselectivity of PGA(A) was predicted. (C) 2015 Elsevier B.V. All rights reserved.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2016

Počet záznamů: 1