Počet záznamů: 1
TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro
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SYSNO ASEP 0454970 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro Tvůrce(i) Tomaštíková, Eva (UEB-Q) RID, ORCID
Demidov, D. (DE)
Jeřábková, Hana (UEB-Q) RID, ORCID
Binarová, Pavla (MBU-M) RID, ORCID
Houben, A. (DE)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Petrovská, Beáta (UEB-Q) RID, ORCIDZdroj.dok. Plant Molecular Biology Reporter - ISSN 0735-9640
Roč. 33, č. 6 (2015), s. 1988-1995Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Aurora kinase ; Targeting protein for Xklp2 ; In vitro kinase assay Vědní obor RIV EB - Genetika a molekulární biologie CEP GA14-28443S GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP501/12/2333 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; MBU-M - RVO:61388971 UT WOS 000367464000031 DOI 10.1007/s11105-015-0890-x Anotace Aurora kinases are involved in various mitotic events, including chromosome segregation and bipolar mitotic spindle assembly. In animals, Aurora A is activated and protected by microtubule-associated protein TPX2. Such role in plants is not known. Here, we have assessed the ability of TPX2 of Arabidopsis to regulate Aurora family members in vitro. AtTPX2 acts as substrate as well as activator of AtAurora1, but not AtAurora3. Truncated version of AtTPX2 lacking the Aurora binding domain is unable to activate the kinases; however, it is still phosphorylated. AtTPX2-induced activation of AtAurora1 results in a dramatically increased phosphorylation level of downstream targets, particularly histone H3. The differences in the activation mechanism of AtAurora1 and 3 point to a specific regulation of both kinases, which may play an important role in cell cycle regulation and signaling cascade transduction in plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2016
Počet záznamů: 1