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Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells
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SYSNO ASEP 0453400 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells Tvůrce(i) Lieskovská, Jaroslava (BC-A) RID
Páleníková, Jana (BC-A)
Širmarová, J. (CZ)
Elsterová, Jana (BC-A) ORCID
Kotsyfakis, Michalis (BC-A) RID, ORCID
Chagas, A. C. (US)
Calvo, E. (US)
Růžek, Daniel (BC-A) RID, ORCID
Kopecký, Jan (BC-A) RIDZdroj.dok. Parasite immunology. - : Wiley - ISSN 0141-9838
Roč. 37, č. 2 (2015), s. 70-78Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Tick ; Dendritic cells ; Interferon ; Cystatin Vědní obor RIV EC - Imunologie CEP GAP302/12/2208 GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000348718100002 DOI 10.1111/pim.12162 Anotace Type I interferon (IFN), mainly produced by dendritic cells (DCs), is critical in the host defence against tick-transmitted pathogens. Here, we report that salivary cysteine protease inhibitor from the hard tick Ixodes scapularis, sialostatin L2, affects IFN- mediated immune reactions in mouse dendritic cells. Following IFN receptor ligation, the Janus activated kinases/signal transducer and activator of transcription (JAK/STAT) pathway is activated. We show that sialostatin L2 attenuates phosphorylation of STATs in spleen dendritic cells upon addition of recombinant IFN-. LPS-stimulated dendritic cells release IFN- which in turn leads to the induction of IFN-stimulated genes (ISG) through JAK/STAT pathway activation. The induction of two ISG, interferon regulatory factor 7 (IRF-7) and IP-10, was suppressed by sialostatin L2 in LPS-stimulated dendritic cells. Finally, the interference of sialostatin L2 with IFN action led to the enhanced replication of tick-borne encephalitis virus in DC. In summary, we present here that tick salivary cystatin negatively affects IFN- responses which may consequently increase the pathogen load after transmission via tick saliva. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
Počet záznamů: 1