Počet záznamů: 1

Highly divergent 18S rRNA gene paralogs in a Cryptosporidium genotype from eastern chipmunks (Tamias striatus)

SYSNO ASEP	0453300
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Highly divergent 18S rRNA gene paralogs in a Cryptosporidium genotype from eastern chipmunks (Tamias striatus)
Tvůrce(i)	Stenger, B.L.S. (US) Clark, M.E. (US) Kváč, Martin (BC-A)_{RID, RID, ORCID} Khan, E. (US) Giddings, C.W. (US) Dyer, N.W. (US) Schultz, J.L. (US) McEvoy, J.M. (US)
Zdroj.dok.	Infection, Genetics and Evolution. - : Elsevier - ISSN 1567-1348 Roč. 32, JUN 2015 (2015), s. 113-123
Poč.str.	11 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Cryptosporidium ; Paralogy ; 18S rRNA ; 18S rDNA
Vědní obor RIV	GJ - Choroby a škůdci zvířat, veterinární medicína
CEP	LH11061 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000355027300015
DOI	10.1016/j.meegid.2015.03.003
Anotace	Cryptosporidium is an apicomplexan parasite that causes the disease cryptosporidiosis in humans, livestock, and other vertebrates. Much of the knowledge on Oyptosporidium diversity is derived from 18S rRNA gene (18S rDNA) phylogenies. Eukaryote genomes generally have multiple 18S rDNA copies that evolve in concert, which is necessary for the accurate inference of phylogenetic relationships. However, 18S rDNA copies in some genomes evolve by a birth-and-death process that can result in sequence divergence among copies. Most notably, divergent 18S rDNA paralogs in the apicomplexan Plasmodium share only 89-95% sequence similarity, encode structurally distinct rRNA molecules, and are expressed at different life cycle stages. In the present study, Cryptosporidium 18S rDNA was amplified from 28/72 (38.9%) eastern chipmunks (Tamias striatus). Phylogenetic analyses showed the co-occurrence of two 18S rDNA types, Type A and Type B, in 26 chipmunks, and Type B clustered with a sequence previously identified as Cryptosporidium chipmunk genotype II. Types A and B had a sister group relationship but shared less than 93% sequence similarity. In contrast, actin and heat shock protein 70 gene sequences were homogeneous in samples with both Types A and B present. It was therefore concluded that Types A and B are divergent 18S rDNA paralogs in Ciyptosporidium chipmunk genotype II. Substitution patterns in Types A and B were consistent with functionally constrained evolution; however, Type B evolved more rapidly than Type A and had a higher G + C content (46.3% versus 41.0%). Oocysts of Oyptosporidium chipmunk genotype II measured 4.17 mu m (3.73-5.04 mu m) x 3.94 mu m (3.50-4.98 mu m) with a length-to-width ratio of 1.06 +/- 0.06 mu m, and infection occurred naturally in the jejunum, cecum, and colon of eastern chipmunks. The findings of this study have implications for the use of 18S rDNA sequences to infer phylogenetic relationships.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2016

Počet záznamů: 1