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The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
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SYSNO ASEP 0451947 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells Tvůrce(i) Filová, Elena (FGU-C) RID, ORCID
Fojt, J. (CZ)
Krýslová, Markéta (FGU-C)
Moravec, H. (CZ)
Joska, L. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDZdroj.dok. International Journal of Nanomedicine. - : Dove Medical Press
Roč. 10, 20 Nov (2015), s. 7145-7163Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. NZ - Nový Zéland Klíč. slova nanostructure ; titanium nanotubes ; cell adhesion ; Saos-2 cells ; osteogenic differentiation Vědní obor RIV EI - Biotechnologie a bionika CEP NT13297 GA MZd - Ministerstvo zdravotnictví GA15-01558S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000365196500001 EID SCOPUS 84948704216 DOI 10.2147/IJN.S87474 Anotace Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2016
Počet záznamů: 1