Počet záznamů: 1  

Tribolium castaneum defensins are primarily active against Gram-positive bacteria

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    SYSNO ASEP0451350
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevTribolium castaneum defensins are primarily active against Gram-positive bacteria
    Tvůrce(i) Tonk, M. (DE)
    Knorr, E. (DE)
    Cabezas-Cruz, A. (FR)
    Valdés, James J. (BC-A) RID
    Kollewe, C. (DE)
    Vilcinskas, A. (DE)
    Zdroj.dok.Journal of Invertebrate Pathology. - : Elsevier - ISSN 0022-2011
    Roč. 132, NOV 2015 (2015), s. 208-215
    Poč.str.8 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaAntimicrobial peptides ; Defensin ; Innate immunity ; Insects ; Tribolium castaneum ; Gram-positive bacteria
    Vědní obor RIVEI - Biotechnologie a bionika
    CEPEE2.3.30.0032 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000365630200023
    DOI10.1016/j.jip.2015.10.009
    AnotaceThe red flour beetle Tribolium castaneum is a destructive insect pest of stored food and feed products, and a model organism for development, evolutionary biology and immunity. The insect innate immune system includes antimicrobial peptides (AMPs) with a wide spectrum of targets including viruses, bacteria, fungi and parasites. Defensins are an evolutionarily-conserved class of AMPs and a potential new source of antimicrobial agents. In this context, we report the antimicrobial activity, phylogenetic and structural properties of three T. castaneum defensins (Def1, Def2 and Def3) and their relevance in the immunity of T. castaneum against bacterial pathogens. All three recombinant defensins showed bactericidal activity against Micrococcus luteus and Bacillus thuringiensis serovar tolworthi, but only Def1 and Def2 showed a bacteriostatic effect against Staphylococcus epidermidis. None of the defensins showed activity against the Gram-negative bacteria Escherichia coli and Pseudomonas entomophila or against the yeast Saccharomyces cerevisiae. All three defensins were transcriptionally upregulated following a bacterial challenge, suggesting a key role in the immunity of T. castaneum against bacterial pathogens. Phylogenetic analysis showed that defensins from T. castaneum, mealworms, Udo longhorn beetle and houseflies cluster within a well-defined clade of insect defensins. We conclude that T. castaneum defensins are primarily active against Gram-positive bacteria and that other AMPs may play a more prominent role against Gram-negative species.
    PracovištěBiologické centrum (od r. 2006)
    KontaktVáclava Lavičková, lavickova@hbu.cas.cz, Tel.: 387 775 889
    Rok sběru2016