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Tribolium castaneum defensins are primarily active against Gram-positive bacteria
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SYSNO ASEP 0451350 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tribolium castaneum defensins are primarily active against Gram-positive bacteria Tvůrce(i) Tonk, M. (DE)
Knorr, E. (DE)
Cabezas-Cruz, A. (FR)
Valdés, James J. (BC-A) RID, ORCID
Kollewe, C. (DE)
Vilcinskas, A. (DE)Zdroj.dok. Journal of Invertebrate Pathology. - : Elsevier - ISSN 0022-2011
Roč. 132, NOV 2015 (2015), s. 208-215Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Antimicrobial peptides ; Defensin ; Innate immunity ; Insects ; Tribolium castaneum ; Gram-positive bacteria Vědní obor RIV EI - Biotechnologie a bionika CEP EE2.3.30.0032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 UT WOS 000365630200023 DOI 10.1016/j.jip.2015.10.009 Anotace The red flour beetle Tribolium castaneum is a destructive insect pest of stored food and feed products, and a model organism for development, evolutionary biology and immunity. The insect innate immune system includes antimicrobial peptides (AMPs) with a wide spectrum of targets including viruses, bacteria, fungi and parasites. Defensins are an evolutionarily-conserved class of AMPs and a potential new source of antimicrobial agents. In this context, we report the antimicrobial activity, phylogenetic and structural properties of three T. castaneum defensins (Def1, Def2 and Def3) and their relevance in the immunity of T. castaneum against bacterial pathogens. All three recombinant defensins showed bactericidal activity against Micrococcus luteus and Bacillus thuringiensis serovar tolworthi, but only Def1 and Def2 showed a bacteriostatic effect against Staphylococcus epidermidis. None of the defensins showed activity against the Gram-negative bacteria Escherichia coli and Pseudomonas entomophila or against the yeast Saccharomyces cerevisiae. All three defensins were transcriptionally upregulated following a bacterial challenge, suggesting a key role in the immunity of T. castaneum against bacterial pathogens. Phylogenetic analysis showed that defensins from T. castaneum, mealworms, Udo longhorn beetle and houseflies cluster within a well-defined clade of insect defensins. We conclude that T. castaneum defensins are primarily active against Gram-positive bacteria and that other AMPs may play a more prominent role against Gram-negative species. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
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