Počet záznamů: 1
Ionizing radiation increases primary cilia incidence and induces multiciliation in C2C12 myoblasts
- 1.
SYSNO ASEP 0451039 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ionizing radiation increases primary cilia incidence and induces multiciliation in C2C12 myoblasts Tvůrce(i) Filipová, A. (CZ)
Diaz-Garcia, D. (CZ)
Bezrouk, A. (CZ)
Čížková, D. (CZ)
Havelek, R. (CZ)
Vávrová, J. (CZ)
Dayanithi, Govindan (UEM-P) RID
Řezáčová, M. (CZ)Celkový počet autorů 8 Zdroj.dok. Cell Biology International. - : Elsevier - ISSN 1065-6995
Roč. 39, č. 8 (2015), s. 943-953Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova cell line ; ionizing radiation ; multiple cilia ; myoblast ; primary cilium ; serum starvation stress Vědní obor RIV FP - Ostatní lékařské obory Institucionální podpora UEM-P - RVO:68378041 UT WOS 000358454300007 EID SCOPUS 84937641870 DOI 10.1002/cbin.10462 Anotace Primary cilia act as physical-chemical sensors and their functions include the perception of the extracellular milieu, regulation of organogenesis, and cell polarity. In general, these cells are monociliated and the single cilium possesses diverse receptors and channels which are involved in morphogenesis and growth signaling, and are, therefore, important for cell proliferation and differentiation. In this study, we used an in vitro model of C2C12 myoblasts to evaluate the effect of DNA damage induced by gamma ionizing radiation on primary cilia incidence. A significantly higher number of ciliated cells were observed after 1 day post-irradiation with 2-20 Gy when compared with non-irradiated cells. After 3 days post-irradiation, the cilia incidence in cells had decreased slightly when treated with 2, 6, and 10 Gy, although an increase in incidence rate was observed in cells treated with 20 Gy. Multi-ciliated cells were also detected in myoblasts irradiated with 10 and 20 Gy but not in non-irradiated cells or after low irradiation (2-6 Gy). Irradiation also caused a dose-dependent decrease in cell viability and proliferation and corresponding cell cycle arrest. Furthermore, an activation of caspases 3/7, 8, and 9 was observed after higher radiation (10 and 20 Gy) with increased apoptosis. Together, our results show that irradiation by gamma rays promotes myoblast ciliogenesis, with pronounced effects observed after 3 days post-irradiation. We conclude that irradiation doses of 10 and 20 Gy are sufficient to induce cell death and are responsible for the formation of multiple cilia originating from multiple basal bodies. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2016
Počet záznamů: 1