Počet záznamů: 1
Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface
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SYSNO ASEP 0450679 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface Tvůrce(i) Hammoudi, P.-M. (CH)
Jacot, D. (CH)
Mueller, C. (CH)
Di Cristina, M. (IT)
Dogga, S.K. (CH)
Marq, J.-B. (CH)
Romano, J. (US)
Tosetti, N. (CH)
Dubrot, J. (CH)
Emre, Y. (CH)
Lunghi, M. (IT)
Coppens, I. (US)
Yamamoto, M. (JP)
Sojka, Daniel (BC-A) RID, ORCID
Pino, P. (CH)
Soldati-Favre, D. (CH)Zdroj.dok. PLoS Pathogens. - : Public Library of Science - ISSN 1553-7366
Roč. 11, č. 10 (2015), e1005211Poč.str. 32 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Toxoplasma gondii ; Plasmodium proteins ; ASP5 Vědní obor RIV EB - Genetika a molekulární biologie Institucionální podpora BC-A - RVO:60077344 UT WOS 000364462700041 DOI 10.1371/journal.ppat.1005211 Anotace Toxoplasma gondii possesses sets of dense granule proteins (GRAs) that either assemble at, or cross the parasitophorous vacuole membrane (PVM) and exhibit motifs resembling the HT/PEXEL previously identified in a repertoire of exported Plasmodium proteins. Within Plasmodium spp., cleavage of the HT/PEXEL motif by the endoplasmic reticulum-resident protease Plasmepsin V precedes trafficking to and export across the PVM of proteins involved in pathogenicity and host cell remodelling. Here, we have functionally characterized the T. gondii aspartyl protease 5 (ASP5), a Golgi-resident protease that is phylogenetically related to Plasmepsin V. We show that deletion of ASP5 causes a significant loss in parasite fitness in vitro and an altered virulence in vivo. Furthermore, we reveal that ASP5 is necessary for the cleavage of GRA16, GRA19 and GRA20 at the PEXEL-like motif. In the absence of ASP5, the intravacuolar nanotubular network disappears and several GRAs fail to localize to the PVM, while GRA16 and GRA24, both known to be targeted to the host cell nucleus, are retained within the vacuolar space. Additionally, hypermigration of dendritic cells and bradyzoite cyst wall formation are impaired, critically impacting on parasite dissemination and persistence. Overall, the absence of ASP5 dramatically compromises the parasite's ability to modulate host signalling pathways and immune responses. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
Počet záznamů: 1