Počet záznamů: 1
Radiolabeling of antibody for epitope of human carbonic anhydrase IX (IgG M75) by 61Cu and 64Cu and its biological testing
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SYSNO ASEP 0450524 Druh ASEP A - Abstrakt Zařazení RIV O - Ostatní Název Radiolabeling of antibody for epitope of human carbonic anhydrase IX (IgG M75) by 61Cu and 64Cu and its biological testing Tvůrce(i) Čepa, Adam (UJF-V)
Ráliš, Jan (UJF-V) SAI
Pavelka, A. (CZ)
Marešová, L. (CZ)
Kleinová, M. (CZ)
Seifert, Daniel (UJF-V) ORCID
Sieglová, Irena (UMG-J)
Král, Vlastimil (UMG-J) RID
Polášek, Miloslav (UJF-V)
Lebeda, Ondřej (UJF-V) RID, ORCID, SAI
Paúrová, M. (CZ)
Lázníček, M. (CZ)Celkový počet autorů 12 Zdroj.dok. European Journal of Nuclear Medicine and Molecular Imaging. - : Springer - ISSN 1619-7070
Roč. 42, S (2015), s. 465-466Poč.str. 2 s. Forma vydání Tištěná - P Akce 28th Annual congress of the European-Association-of-Nuclear-Medicine (EANM) Datum konání 10.10.2015-14.10.2015 Místo konání Hamburg Země DE - Německo Typ akce EUR Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova antibodies ; Cu-61 ; Cu-64 Vědní obor RIV BG - Jaderná, atomová a mol. fyzika, urychlovače Vědní obor RIV – spolupráce Ústav molekulární genetiky - Genetika a molekulární biologie CEP TA02010797 GA TA ČR - Technologická agentura ČR LM2011019 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UJF-V - RVO:61389005 ; UMG-J - RVO:68378050 UT WOS 000363013202432 Anotace Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells. The antibody IgG M75 was successfully conjugated with a new, copper-specific chelator “phosphinate”, the conjugation method was optimized and is well-reproducible. The conjugate was then labelled with two positron emitting copper radioisotopes, Cu-61 (3.333 h) and Cu-64 (12.701 h). Labelled conjugates were tested in vivo in mice with colorectal cancer xenografts. Copper radioisotopes were produced via Ni-nat(d,x) and Ni-64(p,n) reactions (Ni, 99.999 %, Goodfellow; Ni-64, 99.09 %, Isoflex). Specific activity of Cu-61 was 1 GBq/mg Cu and more, which was satisfactory for the labelling purposes. All reagents were analytical grade and were purified prior labelling with chelex-100 in sodium form, 100-200 mesh from metals surplus. Chelator “phospinate” was synthesized and characterized in our laboratories. Antibody IgG M75 was prepared by genetic engineering methods. Immunoconjugates (IgG M75-SCN-phospinate) were separated with use of PD 10 Desalting Columns and analyzed using size exclusion chromatography column BioSep SEC-S-3000. The conjugate M75-SCN-phosphinate was labelled successfully with both Cu-61 (96% yield) and Cu-64 (97% yield). Biodistribution experiments revealed high uptake of both labelled compounds in tumour tissue: Cu-61-labelled conjugate showed uptake of about 10 % ID/g after 6 hours, while Cu-64-labeled about 24 % ID/g even after 48 hours. The labelling resulted in the product of high specific activity (1.0 and 7.4 MBq/microgram, respectively) and of high radiochemical purity (> 95 %). The labelled molecule has considerable potential as a radioimmunopharmaceutical suitable for imaging of tumors expressing carbonic anhydrase IX by positron emission tomography. Pracoviště Ústav jaderné fyziky Kontakt Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Rok sběru 2016
Počet záznamů: 1