Proton-induced direct and indirect damage of plasmid DNA

Vyšín, Luděk; Pachnerová Brabcová, Kateřina; Štěpán, V.; Moretto-Capelle, P.; Bugler, B.; Legube, G.; Cafarelli, P.; Casta, R.; Champeaux, J. P.; Sence, M.; Vlk, M.; Wagner, Richard; Štursa, Jan; Zach, Václav; Incerti, S.; Juha, Libor; Davídková, Marie

doi:https://dx.doi.org/10.1007/s00411-015-0605-6

Počet záznamů: 1

Proton-induced direct and indirect damage of plasmid DNA

1.

SYSNO ASEP	0448614
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Proton-induced direct and indirect damage of plasmid DNA
Tvůrce(i)	Vyšín, Luděk (FZU-D)_{RID, ORCID} Pachnerová Brabcová, Kateřina (UJF-V)_{RID, ORCID, SAI} Štěpán, V. (FR) Moretto-Capelle, P. (FR) Bugler, B. (FR) Legube, G. (FR) Cafarelli, P. (FR) Casta, R. (FR) Champeaux, J. P. (FR) Sence, M. (FR) Vlk, M. (CZ) Wagner, Richard (UJF-V) Štursa, Jan (UJF-V)_{RID, ORCID} Zach, Václav (UJF-V)_{SAI, ORCID} Incerti, S. (FR) Juha, Libor (FZU-D)_{RID, ORCID, SAI} Davídková, Marie (UJF-V)_{RID, ORCID, SAI}
Zdroj.dok.	Radiation and Environmental Biophysics - ISSN 0301-634X Roč. 54, č. 3 (2015), s. 343-352
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	proton radiation ; DNA plasmid ; direct and indirect effects ; clustered damage ; repair enzymes
Vědní obor RIV	BO - Biofyzika
CEP	GA13-28721S GA ČR - Grantová agentura ČR
	LD12008 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LM2011019 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	FZU-D - RVO:68378271 ; UJF-V - RVO:61389005
UT WOS	000358328800010
EID SCOPUS	84938996562
DOI	10.1007/s00411-015-0605-6
Anotace	Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.
Pracoviště	Fyzikální ústav
Kontakt	Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
Rok sběru	2016

Počet záznamů: 1