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Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum
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SYSNO ASEP 0448547 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum Tvůrce(i) Nováková, Eva (FZU-D)
Vyšín, Luděk (FZU-D) RID, ORCID
Burian, Tomáš (FZU-D) RID, ORCID
Juha, Libor (FZU-D) RID, ORCID, SAI
Davídková, Marie (UJF-V) RID, ORCID, SAI
Múčka, V. (CZ)
Čuba, V. (CZ)
Grisham, M. E. (US)
Heinbuch, S. (US)
Rocca, J.J. (US)Zdroj.dok. Physical Review E. - : American Physical Society - ISSN 1539-3755
Roč. 91, č. 4 (2015), "042718-1"-"042718-8"Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova XUV ; DNA damages ; single-strand breaks (SSBs) ; double-strand breaks (DSBs) Vědní obor RIV BO - Biofyzika CEP GBP108/12/G108 GA ČR - Grantová agentura ČR GA13-28721S GA ČR - Grantová agentura ČR Institucionální podpora FZU-D - RVO:68378271 ; UJF-V - RVO:61389005 UT WOS 000353637500008 EID SCOPUS 84929192771 DOI 10.1103/PhysRevE.91.042718 Anotace Ionizing radiation induces a variety of DNA damages including single-strand breaks (SSBs), double-strand breaks (DSBs), abasic sites, modified sugars, and bases. We have studied the nature of DNA damage induced directly by the pulsed 46.9nm (26.5 eV) radiation provided by an extreme ultraviolet (XUV) capillary-discharge Ne-like Ar laser (CDL). Doses up to 45 kGy were delivered with a repetition rate of 3 Hz. We studied the dependence of the yield of SSBs and DSBs of a simple model of DNA molecule (pBR322) on the CDL pulse fluence. Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. The action cross sections of the single and double-strand breaks of pBR322 plasmid DNA in solid state were determined. We observed an increase in the efficiency of strand-break induction in the supercoiled DNA as a function of laser pulse fluence. Results are compared to those acquired at synchrotron radiation facilities and other sources of XUV and soft x-ray radiation. Pracoviště Fyzikální ústav Kontakt Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Rok sběru 2016
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