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Nanotherapeutics shielded with a pH responsive polymeric layer

SYSNO ASEP	0448519
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Nanotherapeutics shielded with a pH responsive polymeric layer
Tvůrce(i)	Kostka, Libor (UMCH-V)_{RID, ORCID} Šubr, Vladimír (UMCH-V)_{RID, ORCID} Laga, Richard (UMCH-V)_{RID, ORCID} Chytil, Petr (UMCH-V)_{RID, ORCID} Ulbrich, Karel (UMCH-V)_RID Seymour, L. W. (GB) Etrych, Tomáš (UMCH-V)_{RID, ORCID}
Zdroj.dok.	Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408 Roč. 64, Suppl. 1 (2015), S29-S40
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	nanotherapeutics ; coating ; pH responsive
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	EE2.3.30.0029 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GAP301/12/1254 GA ČR - Grantová agentura ČR
Institucionální podpora	UMCH-V - RVO:61389013
UT WOS	000365010700005
EID SCOPUS	84952656462
Anotace	Efficient intravenous delivery is the greatest single hurdle, with most nanotherapeutics frequently found to be unstable in the harsh conditions of the bloodstream. In the case of nanotherapeutics for gene delivery, viral vectors are often avidly recognized by both the innate and the adaptive immune systems. So, most modern delivery systems have benefited from being coated with hydrophilic polymers. Self-assembling delivery systems can achieve both steric and lateral stabilization following surface coating, endowing them with much improved systemic circulation properties and better access to disseminated targets; similarly, gene delivery viral vectors can be ‘stealthed’ and their physical properties modulated by surface coating. Polymers that start degrading under acidic conditions are increasingly investigated as a pathway to trigger the release of drugs or genes once the carrier reaches a slightly acidic tumor environment or after the carrier has been taken up by cells, resulting in the localization of the polymer in acidic endosomes and lysosomes. Advances in the design of acid-degradable drug and gene delivery systems have been focused and discussed in this article with stress placed on HPMA-based copolymers. We designed a system that is able to “throw away” the polymer coat after successful transport of the vector into a target cell. Initial biological studies were performed and it was demonstrated that this principle is applicable for real adenoviral vectors. It was shown that the transfection ability of coated virus at pH 7.4 is 75 times lower then transfection at pH 5.4.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2016
Elektronická adresa	http://www.biomed.cas.cz/physiolres/pdf/64%20Suppl%201/64_S29.pdf

Počet záznamů: 1