Počet záznamů: 1
Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics
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SYSNO ASEP 0447841 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics Tvůrce(i) Loizeau, D. (FR)
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Aydogan, Gokcan (UFCH-W)
Philimonenko, Anatoly (UMG-J)
Mahfoudhi, S. (FR)
Hozák, Pavel (UMG-J) RID, ORCID
Maroto, A. (FR)
Couthon-Courves, H. (FR)
Jaffres, P.-A. (FR)
Deschamps, L. (FR)
Giamarchi, P. (FR)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
Roč. 136, DEC 2015 (2015), s. 192-200Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova gene therapy ; transfection ; non-viral DNA vector Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Vědní obor RIV – spolupráce Ústav molekulární genetiky - Genetika a molekulární biologie CEP GBP208/12/G016 GA ČR - Grantová agentura ČR Institucionální podpora UFCH-W - RVO:61388955 ; UMG-J - RVO:68378050 UT WOS 000367408100025 EID SCOPUS 84941945136 DOI 10.1016/j.colsurfb.2015.09.012 Anotace Cationic lipids are used to deliver genetic material to living cells. Their proper biophysical characterization is needed in order to design and control this process. In the present work we characterize some properties of recently synthetized cationic lipophosphoramidates. The studied compounds share the same structure of their hydrophobic backbone, but differ in their hydrophilic cationic headgroup, which is formed by a trimethylammonium, a trimethylarsonium or a dicationic moiety. Dynamic light scattering and cryo-transmission electron microscopy proves that the studied lipophosphoramidates create stable unilamellar vesicles. Fluorescence of polarity probe, Laurdan, analyzed using time-dependent fluorescence shift method (TDFS) and generalized polarization (GP) gives important information about the phase, hydration and dynamics of the lipophosphoramidate bilayers. While all of the compounds produced lipid bilayers that were sufficiently fluid for their potential application in gene therapy, their polarity/hydration and mobility was lower than for the standard cationic lipid – DOTAP. Mixing cationic lipophosphoramidates with DOPC helps to reduce this difference. The structure of the cationic headgroup has an important and complex influence on bilayer hydration and mobility. Both TDFS and GP methods are suitable for the characterization of cationic amphiphiles and can be used for screening of the newly synthesized compounds. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2016
Počet záznamů: 1