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Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating
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SYSNO ASEP 0446216 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating Tvůrce(i) Huk, A. (NO)
Izak-Nau, E. (AT)
el Yamani, N. (NO)
Uggerud, H. (NO)
Vadset, M. (NO)
Zasońska, Beata Anna (UMCH-V) RID, ORCID
Duschl, A. (AT)
Dusinska, M. (NO)Zdroj.dok. Particle and Fibre Toxicology. - : BioMed Central - ISSN 1743-8977
Roč. 12, 24 July (2015), 25_1-25_20Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova silver nanomaterials ; surface charge ; surface coating Vědní obor RIV CD - Makromolekulární chemie Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000358406900001 EID SCOPUS 84937797469 DOI https://doi.org/10.1186/s12989-015-0100-x Anotace The main goal of this research was to study the interactions of a fully characterized set of silver nanomaterials (Ag ENMs) with cells in vitro, according to the standards of Good Laboratory Practices (GLP), to assure the quality of nanotoxicology research. We were interested in whether Ag ENMs synthesized by the same method, with the same size distribution, shape and specific surface area, but with different charges and surface compositions could give different biological responses. A range of methods and toxicity endpoints were applied to study the impacts of interaction of the Ag ENMs with TK6 cells. As tests of viability, relative growth activity and trypan blue exclusion were applied. Genotoxicity was evaluated by the alkaline comet assay for detection of strand breaks and oxidized purines. The mutagenic potential of Ag ENMs was investigated with the in vitro HPRT gene mutation test on V79-4 cells according to the OECD protocol. Ag ENM agglomeration, dissolution as well as uptake and distribution within the cells were investigated as crucial aspects of Ag ENM toxicity. Ag ENM stabilizers were included in addition to positive and negative controls. Different cytotoxic effects were observed including membrane damage, cell cycle arrest and cell death. Ag ENMs also induced various kinds of DNA damage including strand breaks and DNA oxidation, and caused gene mutation. We found that positive Ag ENMs had greater impact on cyto- and genotoxicity than did Ag ENMs with neutral or negative charge, assumed to be related to their greater uptake into cells and to their presence in the nucleus and mitochondria, implying that Ag ENMs might induce toxicity by both direct and indirect mechanisms. We showed that Ag ENMs could be cytotoxic, genotoxic and mutagenic. Our experiments with the HPRT gene mutation assay demonstrated that surface chemical composition plays a significant role in Ag ENM toxicity. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2016
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