Počet záznamů: 1
Expression of clock genes /period/ and /timeless /in the central nervous system of the mediterranean flour moth, Ephestia kuehniella
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SYSNO ASEP 0444917 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Expression of clock genes /period/ and /timeless /in the central nervous system of the mediterranean flour moth, Ephestia kuehniella Tvůrce(i) Kobelková, Alena (BC-A)
Závodská, Radka (BC-A) RID, ORCID
Šauman, Ivo (BC-A) RID, ORCID
Bazalová, Olga (BC-A) RID
Doležel, David (BC-A) RID, ORCIDCelkový počet autorů 5 Zdroj.dok. Journal of Biological Rhythms. - : Sage - ISSN 0748-7304
Roč. 30, č. 2 (2015), s. 104-116Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova circadian clock ; activity rhythms ; eclosion rhythm Vědní obor RIV EB - Genetika a molekulární biologie CEP GC14-32654J GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000352581100005 EID SCOPUS 84926363243 DOI 10.1177/0748730414568430 Anotace Homologous circadian genes are found in all insect clocks, but their contribution to species-specific circadian timing systems differs. The aim of this study was to extend research within Lepidoptera to gain a better understanding of the molecular mechanism underlying circadian clock plasticity and evolution. The Mediterranean flour moth, Ephestia kuehniella (Pyralidae), represents a phylogenetically ancestral lepidopteran species. We have identified circadian rhythms in egg hatching, adult emergence, and adult locomotor activity. Cloning full-length complementary DNAs and further characterization confirmed one copy of period and timeless genes in both sexes. Both per and tim transcripts oscillate in their abundance in E. kuehniella heads under light-dark conditions. PER-like immunoreactivity (PER-lir) was observed in nuclei and cytoplasm of most neurons in the central brain, the ventral part of subesophageal complex, the neurohemal organs, the optic lobes, and eyes. PER-lir in photoreceptor nuclei oscillated during the day with maximal intensity in the light phase of the photoperiodic regime and lack of a signal in the middle of the dark phase. Expression patterns of per and tim messenger RNAs (mRNAs) were revealed in the identical location as the PER-lir was detected. In the photoreceptors, a daily rhythm in the intensity of expression of both per mRNA and tim mRNA was found. These findings suggest E. kuehniella as a potential lepidopteran model for circadian studies. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
Počet záznamů: 1