Počet záznamů: 1

Transcriptional profiling of Bordetella pertussis reveals requirement of RNA chaperone Hfq for Type III secretion system functionality

SYSNO ASEP	0444487
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Transcriptional profiling of Bordetella pertussis reveals requirement of RNA chaperone Hfq for Type III secretion system functionality
Tvůrce(i)	Bíbová, Ilona (MBU-M)_RID Hot, D. (FR) Keidel, Kristina (MBU-M) Amman, F. (DE) Slupek, S. (FR) Černý, Ondřej (MBU-M) Gross, R. (DE) Večerek, Branislav (MBU-M)_{RID, ORCID}
Zdroj.dok.	RNA biology. - : Taylor & Francis - ISSN 1547-6286 Roč. 12, č. 2 (2015), s. 175-185
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Bsp22 ; Hfq ; infection
Vědní obor RIV	CE - Biochemie
CEP	GAP302/11/1940 GA ČR - Grantová agentura ČR
	EE2.3.20.0055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EE2.3.30.0003 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	7AMB14AR028 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000352238900008
DOI	10.1080/15476286.2015.1017237
Anotace	Bordetella pertussis, the causative agent of human whooping cough (pertussis) produces a complex array of virulence factors in order to establish efficient infection in the host. The RNA chaperone Hfq and small regulatory RNAs are key players in posttranscriptional regulation in bacteria and have been shown to play an essential role in virulence of a broad spectrum of bacterial pathogens. This study represents the first attempt to characterize the Hfq regulon of the human pathogen B. pertussis under laboratory conditions as well as upon passage in the host and indicates that loss of Hfq has a profound effect on gene expression in B. pertussis. Comparative transcriptional profiling revealed that Hfq is required for expression of several virulence factors in B. pertussis cells including the Type III secretion system (T3SS). In striking contrast to the wt strain, T3SS did not become operational in the hfq mutant passaged either through mice or macrophages thereby proving that Hfq is required for the functionality of the B. pertussis T3SS. Likewise, expression of virulence factors vag8 and tcfA encoding autotransporter and tracheal colonization factor, respectively, was strongly reduced in the hfq mutant. Importantly, for the first time we demonstrate that B. pertussis T3SS can be activated upon contact with macrophage cells in vitro.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2016

Počet záznamů: 1