| SYSNO ASEP | 0443963
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| Druh ASEP | J - Článek v odborném periodiku
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| Zařazení RIV | J - Článek v odborném periodiku
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| Poddruh J | Článek ve WOS
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| Název | Influence of Acyclic Nucleoside Phosphonate Antivirals on Gene Expression of Chemokine Receptors CCR5 and CXCR4
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| Tvůrce(i) | Potměšil, P. (CZ)
Holý, Antonín (UOCHB-X)
Zídek, Zdeněk (UEM-P)
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| Celkový počet autorů | 3
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| Zdroj.dok. | Folia Biologica. - : Univerzita Karlova v Praze
- ISSN 0015-5500
Roč. 61, č. 1 (2015), s. 1-7
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| Poč.str. | 7 s.
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| Jazyk dok. | eng - angličtina
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| Země vyd. | CZ - Česká republika
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| Klíč. slova | acyclic nucleoside phosphonate ; HIV ; CCR5 ; CXCR4 ; cytokine ; RT-PCR
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| Vědní obor RIV | CC - Organická chemie
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| Vědní obor RIV – spolupráce | Ústav experimentální medicíny - Farmakologie a lékárnická chemie
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| CEP | GA305/03/1470 GA ČR - Grantová agentura ČR
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| 1M0508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
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| Institucionální podpora | UOCHB-X - RVO:61388963 ; UEM-P - RVO:68378041
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| UT WOS | 000352677300001
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| EID SCOPUS | 84929454062
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| Anotace | Acyclic nucleoside phosphonates (ANPs) are potent antiviral agents effective against replication of DNA viruses and retroviruses including human immunodeficiency virus (HIV). Prototype compound 9-(R)-[2-(phosphonomethoxy)propyl]adenine (tenofovir) is a principal component of drugs widely used in the treatment of HIV infection (Viread, Truvada). Besides their antimetabolic mode of action, ANPs possess immunomodulatory properties. A number of them have been previously found to stimulate secretion of cytokines and anti-HIV effective chemokines. In the present pilot experiments we analysed the in vitro effects of ANPs on the expression of chemokine receptors CCR5 and CXCR4 that are co-receptors of HIV-1 entry in cells. The impact of ANPs was investigated at the level of gene transcription of mRNA in mouse lymphocytes and macrophages using the RT-PCR method. The following compounds were included in the study: 9-(R)-[2-(phosphonomethoxy)propyl]adenine (tenofovir), N-6-cyclopropyl-(R)-9-[2-(phosphonomethoxy)-propyl]2,6-diaminopurine, N-6-cyclopentyl-(R)-9-[2-(phosphonomethoxy)propyl]2,6-diaminopurine, N-6-dimethylaminoethyl-(R)-9-[2-(phosphonomethoxy)propyl]2,6-diaminopurine, N-6-cyclopentyl-9-[2-(phosphonomethoxy)ethyl]2,6-diaminopurine, N-6-isobutyl-9-[2-(phosphonomethoxy)ethyl]2,6-diaminopurine. Gene transcription of chemokine receptors CCR5 and CXCR4 was not affected after application of these acyclic nucleoside phosphonate antivirals.
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| Pracoviště | Ústav organické chemie a biochemie
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| Kontakt | asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
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| Rok sběru | 2016
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