Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity

Turánek Knotigová, P.; Zyka, Daniel; Mašek, J.; Kovalová, Anna; Křupka, M.; Bartheldyová, E.; Kulich, P.; Koudelka, Š.; Lukáč, R.; Kauerová, Z.; Vacek, A.; Stuchlová Horynová, M.; Kozubík, Alois; Miller, A. D.; Fekete, Ladislav; Kratochvílová, Irena; Ježek, Jan; Ledvina, M.; Raška, M.; Turánek, J.

doi:https://dx.doi.org/10.1007/s11095-014-1516-y

Počet záznamů: 1

Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity

1.

SYSNO ASEP	0443903
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity
Tvůrce(i)	Turánek Knotigová, P. (CZ) Zyka, Daniel (UOCHB-X)_RID Mašek, J. (CZ) Kovalová, Anna (UOCHB-X) Křupka, M. (CZ) Bartheldyová, E. (CZ) Kulich, P. (CZ) Koudelka, Š. (CZ) Lukáč, R. (CZ) Kauerová, Z. (CZ) Vacek, A. (CZ) Stuchlová Horynová, M. (CZ) Kozubík, Alois (BFU-R)_{RID, ORCID} Miller, A. D. (GB) Fekete, Ladislav (FZU-D)_{RID, ORCID} Kratochvílová, Irena (FZU-D)_{RID, ORCID, SAI} Ježek, Jan (UOCHB-X)_RID Ledvina, M. (CZ) Raška, M. (CZ) Turánek, J. (CZ)
Celkový počet autorů	20
Zdroj.dok.	Pharmaceutical Research. - : Springer - ISSN 0724-8741 Roč. 32, č. 4 (2015), s. 1186-1199
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	adjuvant ; bone-marrow radioprotection ; liposome ; muramyl dipeptide ; vaccine
Vědní obor RIV	CC - Organická chemie
Vědní obor RIV – spolupráce	Fyzikální ústav - Fyzikální chemie a teoretická chemie Biofyzikální ústav - Biofyzika
CEP	GAP304/10/1951 GA ČR - Grantová agentura ČR
	TA01011165 GA TA ČR - Technologická agentura ČR
Institucionální podpora	UOCHB-X - RVO:61388963 ; BFU-R - RVO:68081707 ; FZU-D - RVO:68378271
UT WOS	000351169800002
EID SCOPUS	84925507869
DOI	10.1007/s11095-014-1516-y
Anotace	The aim of this work was to demonstrate an immunostimulatory and adjuvant effect of new apyrogenic lipophilic derivatives of norAbuMDP and norAbuGMDP formulated in nanoliposomes. Nanoliposomes and metallochelating nanoliposomes were prepared by lipid film hydration and extrusion methods. The structure of the liposomal formulation was studied by electron microscopy, AF microscopy, and dynamic light scattering. Sublethal and lethal gamma-irradiation mice models were used to demonstrate stimulation of innate immune system. Recombinant Hsp90 antigen (Candida albicans) bound onto metallochelating nanoliposomes was used for immunisation of mice to demonstrate adjuvant activities of tested compounds. Safety and stimulation of innate and adaptive immunity were demonstrated on rabbits and mice. The liposomal formulation of norAbuMDP/GMDP was apyrogenic in rabbit test and lacking any side effect in vivo. Recovery of bone marrow after sublethal gamma-irradiation as well as increased survival of mice after lethal irradiation was demonstrated. Enhancement of specific immune response was demonstrated for some derivatives incorporated in metallochelating nanoliposomes with recombinant Hsp90 protein antigen. Liposomal formulations of new lipophilic derivatives of norAbuMDP/GMDP proved themselves as promising adjuvants for recombinant vaccines as well as immunomodulators for stimulation of innate immunity and bone-marrow recovery after chemo/radio therapy of cancer.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2016

Počet záznamů: 1