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Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes

SYSNO ASEP	0441646
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes
Tvůrce(i)	Blaha, Milan (UZFG-Y)_RID Němcová, Lucie (UZFG-Y)_RID Procházka, Radek (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Reproductive Biology and Endocrinology. - : BioMed Central Roč. 13, č. 1 (2015)
Poč.str.	12 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	pig ; oocyte ; cGMP
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GAP502/11/0593 GA ČR - Grantová agentura ČR
	QI101A166 GA MZe - Ministerstvo zemědělství
Institucionální podpora	UZFG-Y - RVO:67985904
UT WOS	000348477500001
DOI	10.1186/1477-7827-13-1
Anotace	Background: Recent results indicate a key role for cyclic guanosine monophosphate (cGMP) in the regulation of oocyte meiotic arrest in preovulatory mammalian follicles. The aim of our study was to determine whether the resumption of oocyte meiosis and expansion of cumulus cells in isolated pig cumulus-oocyte complexes (COCs) can be blocked by a high intracellular concentration of cGMP, and whether this effect is mediated by a cGMP-dependent inhibition of mitogen-activated protein kinase 3/1 (MAPK3/1). Results: The COCs expressed NPPC and NPR2, the key components of cGMP synthesis, and produced a large amount of cGMP upon stimulation with exogenous CNP, which lead to a significant (P < 0.05) delay in oocyte meiotic resumption. The COCs also responded to cGMP analogues by inhibiting the resumption of oocyte meiosis. The inhibitory effect of cGMP on meiotic resumption was reversed by stimulating the COCs with FSH. However, high concentration of intracellular cGMP was not able to suppress FSH-induced activation of MAPK3/1 in cumulus cells, cumulus expansion and expression of expansion-related genes (P > 0.05). Conclusions: The findings of this study indicate that high cGMP concentrations inhibit the maturation of pig oocytes in vitro but the inhibitory mechanism does not involve the suppression of MAPK3/1 activation in cumulus cells.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2015

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