Počet záznamů: 1

Focused directed evolution of beta-glucosidases: theoretical versus real effectiveness of a minimal working setup and simple robust screening

SYSNO ASEP	0440786
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Focused directed evolution of beta-glucosidases: theoretical versus real effectiveness of a minimal working setup and simple robust screening
Tvůrce(i)	Mazura, P. (CZ) Filipi, T. (CZ) Souček, P. (CZ) Brzobohatý, Břetislav (BFU-R)_{RID, ORCID}
Celkový počet autorů	4
Zdroj.dok.	Carbohydrate Research. - : Elsevier - ISSN 0008-6215 Roč. 346, č. 2 (2011), s. 238-242
Poč.str.	5 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Directed evolution ; beta-Glucosidase ; Mutagenesis
Vědní obor RIV	BO - Biofyzika
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000287111400011
DOI	10.1016/j.carres.2010.08.016
Anotace	Here we present an optimized procedure to generate amino acid variations at specific site(s) of proteins, followed by a simple one-step screen for mutants with the desired beta-glucosidase activity. The procedure was evaluated by introducing sequence variation into a codon specifying a non-functional variant of the catalytic nucleophile (E401) of the maize beta-glucosidase Zm-p60.1. Observed and theoretically expected frequencies of the four possible variants of the codon and the two possible phenotypes (functional and non-functional) were investigated. Deviations in codon and phenotype frequencies were expressed as a coefficient. This coefficient was then used to estimate the extent of over-sampling, of the mutant library, which would be necessary to compensate for the underrepresentation of some sequences.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2015

Počet záznamů: 1