Počet záznamů: 1

Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

SYSNO ASEP	0440398
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes
Tvůrce(i)	Baloun, Jiří (BFU-R) Nevrtalová, Eva (BFU-R) Kováčová, Viera (BFU-R) Hudzieczek, Vojtěch (BFU-R)_ORCID Čegan, Radim (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID}
Celkový počet autorů	7
Zdroj.dok.	Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617 Roč. 171, č. 13 (2014), s. 1188-1196
Poč.str.	9 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	PT - Portugalsko
Klíč. slova	Copper ; Genes coding ROS-eliminating and Cu-transporting proteins ; RNA-Seq database
Vědní obor RIV	BO - Biofyzika
CEP	GBP501/12/G090 GA ČR - Grantová agentura ČR
	LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000345629900011
DOI	10.1016/j.jplph.2014.04.014
Anotace	Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2015

Počet záznamů: 1