Počet záznamů: 1
Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes
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SYSNO ASEP 0440398 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes Tvůrce(i) Baloun, Jiří (BFU-R)
Nevrtalová, Eva (BFU-R)
Kováčová, Viera (BFU-R)
Hudzieczek, Vojtěch (BFU-R) ORCID
Čegan, Radim (BFU-R) RID, ORCID
Vyskot, Boris (BFU-R) RID, ORCID
Hobza, Roman (BFU-R) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 171, č. 13 (2014), s. 1188-1196Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. PT - Portugalsko Klíč. slova Copper ; Genes coding ROS-eliminating and Cu-transporting proteins ; RNA-Seq database Vědní obor RIV BO - Biofyzika CEP GBP501/12/G090 GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BFU-R - RVO:68081707 UT WOS 000345629900011 DOI https://doi.org/10.1016/j.jplph.2014.04.014 Anotace Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2015
Počet záznamů: 1