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Plant Cell Morphogenesis: Methods and Protocols
- 1.
SYSNO ASEP 0439872 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Image Analysis: Basic Procedures for Description of Plant Structures Tvůrce(i) Albrechtová, J. (CZ)
Kubínová, Z. (CZ)
Soukup, A. (CZ)
Janáček, Jiří (FGU-C) RID, ORCIDZdroj.dok. Plant Cell Morphogenesis: Methods and Protocols, Vol. 1080. - New York : Humana Press, 2014 / Žárský V. ; Cvrčková F. - ISSN 1064-3745 - ISBN 978-1-62703-643-6 Rozsah stran s. 67-76 Poč.str. 10 s. Poč.str.knihy 285 Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova experiment design ; image analysis ; stereology Vědní obor RIV EA - Morfologické obory a cytologie CEP GAP501/10/0340 GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000326510400006 EID SCOPUS 84887440757 DOI 10.1007/978-1-62703-643-6_5 Anotace This chapter gives examples of basic procedures of quantification of plant structures with the use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2015
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