Počet záznamů: 1
Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model
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SYSNO ASEP 0438976 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model Tvůrce(i) Zídková, J. (CZ)
Melčová, M. (CZ)
Bartošová, K. (CZ)
Šestáková, Ivana (UFCH-W) RID
Zídek, Václav (FGU-C) RID
Száková, J. (CZ)
Miholová, D. (CZ)
Tlustoš, P. (CZ)Zdroj.dok. Czech Journal of Animal Science. - : Czech Academy of Agricultural Sciences - ISSN 1212-1819
Roč. 59, č. 12 (2014), s. 548-556Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova metal intoxication ; liver ; blood plasma Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP IAA400400806 GA AV ČR - Akademie věd GA13-04580S GA ČR - Grantová agentura ČR Institucionální podpora UFCH-W - RVO:61388955 ; FGU-C - RVO:67985823 UT WOS 000346482200002 EID SCOPUS 84913588103 Anotace The response of different strains of laboratory rats (Rattus norvegicus L.) on both acute (via intraperitoneal injection) and chronic (via drinking water and/or diet) cadmium intoxication was investigated in the model study. The rat strains Long Evans (LE), Spontaneously hypertensive rat (SHR), and Brown Norway (BN) were tested and compared, and total Cd levels and metallothionein (MT) concentrations were determined in the liver of experimental animals. The liver MT concentrations were determined by using adsorptive chronopotentiometry and modified Brdicka reaction and were significantly correlated (r = 0.965) with the total liver Cd content. Moreover, the Cd application resulted in increasing zinc liver contents confirming intensive MT synthesis in the rat liver. In the blood plasma, specific enzymatic activity of glutathione reductase (GR) and glutathione-S-transferase (GST) was determined suggesting increasing activity of GR with the amount of applied Cd for all three strains, whereas ambiguous results have been found for the activity of GST. Therefore, MT concentrations seemed to be more sensitive indicators of the Cd intoxication compared to the assessment of the specific enzymatic activity. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2015
Počet záznamů: 1