Počet záznamů: 1
Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients
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SYSNO ASEP 0435970 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients Tvůrce(i) Kováč, M. (CZ)
Vášková, M. (CZ)
Petráčková, Denisa (MBU-M) RID, ORCID
Pelková, V. (CZ)
Mejstříková, E. (CZ)
Kalina, T. (CZ)
Žaliová, M. (CZ)Zdroj.dok. European Cytokine Network - ISSN 1148-5493
Roč. 25, č. 1 (2014), s. 8-13Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova pediatric acute lymphoblastic leukemia ; bone marrow plasma ; cytokine Vědní obor RIV CE - Biochemie CEP NR9531 GA MZd - Ministerstvo zdravotnictví Institucionální podpora MBU-M - RVO:61388971 UT WOS 000341329400002 DOI 10.1684/ecn.2014.0348 Anotace Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of ALL cells. LBMp (leukemic bone marrow plasma) at the time of ALL diagnosis was compared to control plasma of bone marrow (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The cytokine antibody array enabled simultaneous detection of 79 proteins per sample. Candidate proteins exhibiting significantly different profiles were further analyzed and confirmed by ELISA. mRNAexpression of one of the candidate proteins (TIMP1) was studied using quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The cytokine antibody array experiments identified 23 proteins that differed significantly (p<0.05); of these, two proteins (TIMP1 and LIF) withstood the Bonferroni correction. In contrast, little difference was observed between CBMp and CPBp. At the diagnosis of ALL, changes in the soluble microenvironment are detectable in BM plasma. These changes probably participate in the pathogenesis and/or result from the changes in the cell composition. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1