Počet záznamů: 1

The Effects of Chloroquine and Hydroxychloroquine on Nitric Oxide Production in RAW 264.7 and Bone Marrow-Derived Macrophages

SYSNO ASEP	0435944
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The Effects of Chloroquine and Hydroxychloroquine on Nitric Oxide Production in RAW 264.7 and Bone Marrow-Derived Macrophages
Tvůrce(i)	Perečko, Tomáš (BFU-R)_{RID, ORCID} Kassab, R.B. (EG) Vašíček, Ondřej (BFU-R)_{ORCID, RID} Pekarová, Michaela (BFU-R)_RID Jančinová, V. (SK) Lojek, Antonín (BFU-R)_RID
Celkový počet autorů	6
Zdroj.dok.	Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500 Roč. 60, č. 1 (2014), s. 39-44
Poč.str.	6 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	chloroquine ; cytotoxicity ; hydroxychloroquine
Vědní obor RIV	BO - Biofyzika
CEP	EE2.3.30.0030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000343275800006
Anotace	Chloroquine, an antimalarial drug, can also be used in the regulation of the immune system, e.g. it is used in the treatment of autoimmune diseases. In this study we investigated the effects of chloroquine and its hydroxy-derivative on nitric oxide (NO) production in two different cell types: (i) immortalized mouse macrophage cell line RAW 264.7 and (ii) mouse bone marrow-derived macrophages (BMDM). The cells were treated with different concentrations (1-100 mu M) of chloroquine or hydroxy-chloroquine and stimulated with lipopolysaccharide for 24 h to induce NO production. Measurement of nitrites by the Griess reaction was used to evaluate the production of NO. Expression of inducible NO synthase was evaluated with Western blot and ATP-cytotoxicity test was used to measure the viability of the cells. Our results showed that both chloroquine and its hydroxy-derivative inhibited NO production in both cell types. However, based on the results of LD50 these inhibitory effects of both derivatives were due to their cytotoxicity.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2015

Počet záznamů: 1