Počet záznamů: 1
Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale
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SYSNO ASEP 0435343 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale Tvůrce(i) Čapková, M. (CZ)
Šáchová, Jana (UMG-J)
Strnad, Hynek (UMG-J) RID
Kolář, Michal (UMG-J) RID, ORCID
Hroudová, Miluše (UMG-J) RID
Chovanec, M. (CZ)
Čada, Z. (CZ)
Štefl, M. (CZ)
Valach, J. (CZ)
Kastner, J. (CZ)
Smetana, K. Jr. (CZ)
Plzák, J. (CZ)Zdroj.dok. BioMed Research International. - : Hindawi - ISSN 2314-6133
-, April 23 (2014)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Microarray Analysis ; Head and Neck Squamous Cell Carcinoma ; whole-genome scale Vědní obor RIV EB - Genetika a molekulární biologie CEP NT13488 GA MZd - Ministerstvo zdravotnictví Institucionální podpora UMG-J - RVO:68378050 UT WOS 000335235800001 DOI 10.1155/2014/408683 Anotace With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey's P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2015
Počet záznamů: 1