Počet záznamů: 1
Lin28a Is Dormant, Functional, and Dispensable During Mouse Oocyte-to-Embryo Transition
- 1.
SYSNO ASEP 0434369 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lin28a Is Dormant, Functional, and Dispensable During Mouse Oocyte-to-Embryo Transition Tvůrce(i) Flemr, Matyáš (UMG-J)
Moravec, Martin (UMG-J)
Libová, Veronika (UMG-J)
Sedláček, Radislav (UMG-J) RID
Svoboda, Petr (UMG-J) RIDZdroj.dok. Biology of Reproduction. - : Oxford University Press - ISSN 0006-3363
Roč. 90, č. 6 (2014), s. 1-9Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova early development ; genome activation ; guinea pigs ; Let-7 ; LIN28 ; mice ; microRNA ; oocyte ; rats ; RNAi ; rodents ; transgenic/ knockout model ; voles ; zygote Vědní obor RIV EB - Genetika a molekulární biologie CEP LH13084 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2011032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP305/12/G034 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000341573900007 DOI 10.1095/biolreprod.114.118703 Anotace The oocyte-to-embryo transition (OET) denotes transformation of a highly differentiated oocyte into totipotent blastomeres of the early mammalian embryo. OET depends exclusively on maternal RNAs and proteins accumulated during oocyte growth, which implies importance of post-transcriptional control of gene expression. OET includes replacement of abundant maternal microRNAs (miRNAs), enriched also in differentiated cells and exemplified by the Let-7 family, with embryonic miRNAs common in pluripotent stem cells (the miR-290 family in the mouse). Lin28a and its homolog Lin28b encode RNA-binding proteins, which interfere with Let-7 maturation and facilitate reprogramming of induced pluripotent stem cells. Both Lin28a and Lin28b transcripts are abundant in mouse oocytes. To test the role of maternal expression of Lin28a and Lin28b during oocyte-to-zygote reprogramming, we generated mice with oocyte-specific knockdown of both genes by using transgenic RNA interference. Lin28a and Lin28b are dispensable during oocyte growth because their knockdown has no effect on Let-7a levels in fully grown germinal vesicle (GV)-intact oocytes. Furthermore, transgenic females were fertile and produced healthy offspring, and their overall breeding performance was comparable to that of wild-type mice. At the same time, 2-cell embryos derived from transgenic females showed up-regulation of mature Let-7, suggesting that maternally provided LIN28A and LIN28B function during zygotic genome activation. Consistent with this conclusion is increased translation of Lin28a transcripts upon resumption of meiosis. Our data imply dual repression of Let-7 during OET in the mouse model, the selective suppression of Let-7 biogenesis by Lin28 homologs superimposed on previously reported global suppression of miRNA activity. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2015
Počet záznamů: 1