Počet záznamů: 1
Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles
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SYSNO ASEP 0434302 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles Tvůrce(i) Bláhová, L. (CZ)
Kohoutek, J. (CZ)
Lebedová, J. (CZ)
Bláha, L. (CZ)
Večeřa, Zbyněk (UIACH-O) RID
Buchtová, Marcela (UZFG-Y) RID, ORCID
Míšek, Ivan (UZFG-Y)
Hilscherová, K. (CZ)Celkový počet autorů 8 Zdroj.dok. Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 406, č. 24 (2014), s. 5867-5876Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova LC MS/MS ; nanoparticle ; cadmium oxide Vědní obor RIV CB - Analytická chemie, separace Vědní obor RIV – spolupráce Ústav živočišné fyziologie a genetiky CEP GAP503/11/2315 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904 UT WOS 000341834500013 DOI 10.1007/s00216-014-8033-z Anotace The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2015
Počet záznamů: 1