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The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse spermatozoa
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SYSNO ASEP 0434224 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse spermatozoa Tvůrce(i) Žatecká, Eva (BTO-N)
Castillo, J. (ES)
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Děd, Lukáš (BTO-N) RID
Pěknicová, Jana (BTO-N) RID
Oliva, R. (ES)Celkový počet autorů 7 Zdroj.dok. Andrology - ISSN 2047-2919
Roč. 2, č. 6 (2014), s. 910-917Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova protamines ; spermatozoa ; tetrabromobisphenol A ; TUNAL assay Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP503/12/1834 GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000344328700014 DOI 10.1111/j.2047-2927.2014.00257.x Anotace Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant of increasing concern to human health because of its action as an endocrine disruptor. We have previously demonstrated that TBBPA is able to increase apoptosis of testicular cells and other changes in the first and second generations of mice exposed to TBBPA. However, the potential effects of TBBPA on mouse epididymal spermatozoa have not yet been investigated. Therefore, we initiated this study to determine whether TBBPA exposure could also result in increased DNA fragmentation in epididymal spermatozoa and whether it had an effect on the protamines as the major nuclear proteins. C57Bl/6J mouse pups (n = 10) were exposed to TBBPA (experimental group) during the gestation, lactation, pre-pubertal and pubertal periods up to the age of 70 days as previously described and compared to control mouse pups (n = 10) that were not exposed. The results demonstrate that TBBPA treatment results in a significantly decreased protamine 1/protamine 2 ratio (0.362 vs. 0.494; p < 0.001), increased total protamine/DNA ratio (0.517 vs. 0.324; p < 0.001) and increased number of terminal deoxynucleotidyl transferase dUTP nick end labelling positive spermatozoa (39.5% vs. 21.2%; p < 0.05) observed between TBBPA and control mice respectively. These findings indicate that TBBPA exposure, in addition to the resulting increased sperm DNA damage, also has the potential to alter the epigenetic marking of sperm chromatin through generation of an anomalous content and distribution of protamines. The possibility is now open to study whether the detected altered protamine content and DNA integrity are related to the previously observed second-generation effects upon TBBPA exposure. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
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