Počet záznamů: 1
Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions
- 1.
SYSNO ASEP 0434025 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions Tvůrce(i) Mishra, Anamika (UEK-B) RID
Heyer, A. G. (DE)
Mishra, Kumud (UEK-B) RID, ORCID, SAIZdroj.dok. Plant Methods. - : BioMed Central
Roč. 10, č. 38 (2014)Poč.str. 26 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova high-throughput screening ; chlorophyll a fluorescence transients ; cold tolerance ; cold acclimation ; whole plant ; Arabidopsis thaliana Vědní obor RIV EH - Ekologie - společenstva CEP EE2.3.20.0246 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 7E12047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000345243700003 DOI 10.1186/1746-4811-10-38 Anotace Background: An easy and non-invasive method for measuring plant cold tolerance is highly valuable to instigate research targeting breeding of cold tolerant crops. Traditional methods are labor intensive, time-consuming and thereby of limited value for large scale screening. Here, we have tested the capacity of chlorophyll a fluorescence (ChlF) imaging based methods for the first time on intact whole plants and employed advanced statistical classifiers and feature selection rules for finding combinations of images able to discriminate cold tolerant and cold sensitive plants. Results: ChlF emission from intact whole plant rosettes of nine Arabidopsis thaliana accessions was measured for (1) non-acclimated (NAC, six week old plants grown at room temperature), (2) cold acclimated (AC, NAC plants acclimated at 4 degrees C for two weeks), and (3) sub-zero temperature (ST) treated (STT, AC plants treated at -4 degrees C for 8 h in dark) states. Cold acclimation broadened the slow phase of ChlF transients in cold sensitive (Co, C24, Can and Cvi) A. thaliana accessions. Similar broadening in the slow phase of ChlF transients was observed in cold tolerant (Col, Rsch, and Te) plants following ST treatments. ChlF parameters: maximum quantum yield of PSII photochemistry (F-V/F-M) and fluorescence decrease ratio (R-FD) well categorized the cold sensitive and tolerant plants when measured in STT state. We trained a range of statistical classifiers with the sequence of captured ChlF images and selected a high performing quadratic discriminant classifier (QDC) in combination with sequential forward floating selection (SFFS) feature selection methods and found that linear combination of three images showed a reasonable contrast between cold sensitive and tolerant A. thaliana accessions for AC as well as for STT states. Conclusions: ChlF transients measured for an intact whole plant is important for understanding the impact of cold acclimation on photosynthetic processes. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2016
Počet záznamů: 1