Počet záznamů: 1
Plant Endosomes - Methods and Protocols
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SYSNO ASEP 0433745 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název The use of FM dyes to analyze plant endocytosis Tvůrce(i) Malínská, Kateřina (UEB-Q) RID, ORCID
Jelínková, Adriana (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDZdroj.dok. Plant Endosomes - Methods and Protocols, Vol. 1209. - Totowa : Humana Press, 2014 / Otegui M.S. - ISSN 1064-3745 - ISBN 978-1-4939-1419-7 Rozsah stran s. 1-11 Poč.str. 11 s. Poč.výt. 2000 Poč.str.knihy 283 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Endocytosis ; Endomembranes ; FM 1-43 Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/11/2476 GA ČR - Grantová agentura ČR GPP305/11/P797 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000343085300002 EID SCOPUS 84907316781 DOI 10.1007/978-1-4939-1420-3_1 Anotace FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015 Elektronická adresa http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271
Počet záznamů: 1