Počet záznamů: 1  

Biphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of alpha-D-Glucosides Using Sucrose Phosphorylase

  1. 1.
    SYSNO ASEP0433199
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevBiphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of alpha-D-Glucosides Using Sucrose Phosphorylase
    Tvůrce(i) De Winter, K. (BE)
    Desmet, T. (BE)
    Devlamynck, T. (BE)
    Van Renterghem, L. (BE)
    Verhaeghe, T. (BE)
    Pelantová, Helena (MBU-M) ORCID, RID
    Křen, Vladimír (MBU-M) RID, ORCID
    Soetaert, W. (BE)
    Zdroj.dok.Organic Process Research & Development. - : American Chemical Society - ISSN 1083-6160
    Roč. 18, č. 6 (2014), s. 781-787
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovabiphasic systems ; pyrogallol ; biphasic catalysis
    Vědní obor RIVCE - Biochemie
    CEP7E11011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000337870900015
    DOI https://doi.org/10.1021/op400302b
    AnotaceThanks to its broad acceptor specificity, sucrose phosphorylase (SP) has been exploited for the transfer of glucose to a wide variety of acceptor molecules. Unfortunately, the low affinity (K-m > 1 M) of SP towards these acceptors typically urges the addition of cosolvents, which often either fail to dissolve sufficient substrate or progressively give rise to enzyme inhibition and denaturation. In this work, a buffer/ethyl acetate ratio of 5:3 was identified to be the optimal solvent system, allowing the use of SP in biphasic systems. Careful optimization of the reaction conditions enabled the synthesis of a range of alpha-D-glucosides, such as cinnamyl alpha-D-glucopyranoside, geranyl alpha-D-glucopyranoside, 2-O-alpha-D-glucopyranosyl pyrogallol, and series of alkyl gallyl 4-O-alpha-D-glucopyranosides. The usefulness of biphasic catalysis was further illustrated by comparing the glucosylation of pyrogallol in a cosolvent and biphasic reaction system. The acceptor yield for the former reached only 17.4%, whereas roughly 60% of the initial pyrogallol was converted when using biphasic catalysis
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2015
Počet záznamů: 1  

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