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Red blood cells serve as intravascular carriers of myeloperoxidase
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SYSNO ASEP 0431577 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Red blood cells serve as intravascular carriers of myeloperoxidase Tvůrce(i) Adam, M. (US)
Gajdová, Silvie (BFU-R)
Kolářová, Hana (BFU-R) RID
Kubala, Lukáš (BFU-R) RID, ORCID
Lau, D. (DE)
Geisler, A. (DE)Celkový počet autorů 12 Zdroj.dok. Journal of Molecular and Cellular Cardiology. - : Elsevier - ISSN 0022-2828
Roč. 74, SEP (2014), s. 353-363Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Myeloperoxidase ; Erythrocyte ; Cell membranes Vědní obor RIV BO - Biofyzika CEP GCP305/12/J038 GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000340077900039 DOI 10.1016/j.yjmcc.2014.06.009 Anotace Myeloperoxidase (MPO) is a heme enzyme abundantly expressed in polymorphonuclear neutrophils. MPO is enzymatically capable of catalyzing the generation of reactive oxygen species (ROS) and the consumption of nitric oxide (NO). Thus MPO has both potent microbicidal and, upon binding to the vessel wall, pro-inflammatory properties. Interestingly, MPO - a highly cationic protein - has been shown to bind to both endothelial cells and leukocyte membranes. Given the anionic surface charge of red blood cells, we investigated binding of MPO to erythrocytes. Red blood cells (RBCs) derived from patients with elevated MPO plasma levels showed significantly higher amounts of MPO by flow cytometry and ELISA than healthy controls. Heparin-induced MPO-release from patient-derived RBCs was significantly increased compared to controls. Ex vivo experiments revealed dose and time dependency for MPO-RBC binding, and immunofluorescence staining as well as confocal microscopy localized MPO-RBC interaction to the erythrocyte plasma membrane. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2015
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