Počet záznamů: 1
Counting cell number in situ by quantification of dimethyl sulphide in culture headspace
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SYSNO ASEP 0431395 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Counting cell number in situ by quantification of dimethyl sulphide in culture headspace Tvůrce(i) Chippendale, T. W. E. (GB)
Španěl, Patrik (UFCH-W) RID, ORCID
Smith, D. (GB)
El Haj, A. (GB)Zdroj.dok. Analyst. - : Royal Society of Chemistry - ISSN 0003-2654
Roč. 139, JUL 2014 (2014), s. 4903-4907Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova TUBE MASS-SPECTROMETRY ; SIFT-MS ; VOLATILE COMPOUNDS Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000341303000023 EID SCOPUS 84906691516 DOI 10.1039/C4AN01102C Anotace A novel, non-invasive technique is reported for determining the numbers of cells in a culture by quantifying dimethyl sulphide (DMS) in the culture headspace as produced by the cellular enzymatic reduction of dissolved dimethyl sulphoxide (DMSO). Measured DMS concentrations, as performed using selected ion flow tube mass spectrometry (SIFT-MS), in the headspace of 2D and 3D cultures of four cell lines, viz. HEK293 (kidney), MG63 (bone), hepG2 (liver) and CALU-1 (lung), linearly correlate with starting cell number. Clear differences in the rates of production of DMS by the four cell types in both the 2D and 3D situations are seen. This novel analytical technique for cell enumeration offers a significant contribution to quality assessment across cell-based research and industry, including analysis of large scale culture systems, and for routine cell biology research. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2015
Počet záznamů: 1