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Towards optical detection of plasmopara viticola infection in the field
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SYSNO ASEP 0430375 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Towards optical detection of plasmopara viticola infection in the field Tvůrce(i) Šebela, David (UEK-B) RID, SAI
Olejníčková, Julie (UEK-B) RID, SAI
Sotolář, R. (CZ)
Vrchotová, Naděžda (UEK-B) RID, SAI, ORCID
Tříska, Jan (UEK-B) RID, ORCID, SAIZdroj.dok. Journal of Plant Pathology - ISSN 1125-4653
Roč. 96, č. 2 (2014), s. 309-320Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. IT - Itálie Klíč. slova Downy mildew ; grapevine ; UV-induced fluorescence ; chlorophyll fluorescence ; reflectance ; transresveratrol ; 2,4,6-trihydroxyphenanthrene-2-O-glucoside Vědní obor RIV EH - Ekologie - společenstva CEP ED1.1.00/02.0073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000348436800011 EID SCOPUS 84903944280 DOI 10.4454/JPP.V96I2.037 Anotace Chlorophyll fluorescence, UV-induced fluorescence and reflectance signals of grapevine leaf tissue naturally infected by Plasmopara viticola were inspected in three susceptible grapevine cultivars (Cabernet Sauvignon, Pinot Blanc and Pinot Gris). Distribution of FV/FM parameter over the leaf was found to be effective to discriminate symptomatic and asymptomatic leaf tissue. Reduction of FV/FM parameter ~ 25% was found in all infected leaf spots. Infected leaves also expressed significantly changed chlorophyll fluorescence induction kinetics expressing much slower electron transport rate on donor and acceptor site of photosystem II. Symptomatic leaves emitted high fluorescence signal under UV excitation 320nm, centered at 395nm, emanated mainly from highly fluorescent compound 2,4,6-trihydroxyphenanthrene-2-O- glucoside. Increase of this compound was found in all inspected grapevine varieties, while its concentration was strongly dependent on resveratrol concentration. Concentration of trans- resveratrol ranged in asymptomatic leaves from 0.1μg·g-1 (CS) to 0.2μg·g-1 (PB, PG). Highest increase of trans-resveratrol in infected leaf tissue was found in PB (4.4μg·g-1), followed by CS (1.6μg·g-1) and PG (1.1μg·g-1). 2,4,6-trihydroxyphenanthrene-2-O-glucoside concentrations varied from 0.41 (PB), 0.75 (PG) to 1.01μg·g-1 (CS) in asymmptomatic leaves, since its concentration rapidly increase with severity of infection [71.09 (PB), 79.37 (PG), and 26.14 μg·g- 1 (CS)]. Reflectance vegetation indices SRI R750/R700, (R780-R710)/(R780-R680) and CRI700 were the most powerful to follow changes in chlorophylls and carotenoids contents under Plasmopara viticola infection. Infected leaves also exhibited lower chlorophyll a (~50 %) and carotenoids (~70%) content. Combination of these optical signals can be used as an effective, non-invasive tool for an early detection of Plasmopara viticola in field. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2015
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