Počet záznamů: 1
Enzymatic and inhibiting activity in boar epididymal fluid
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SYSNO ASEP 0428718 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název Enzymatic and inhibiting activity in boar epididymal fluid Tvůrce(i) Davidová, Nina (BTO-N)
Ren, Š. (CZ)
Liberda, J. (CZ)
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCIDCelkový počet autorů 5 Zdroj.dok. Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. - Praha : Biotechnologický ústav AVČR, v.v.i, 2014 / Pěknicová Jana
S. 1-82Poč.str. 2 s. Akce XXth Symposium of Biology and Immunology of Reproduction with international participation Datum konání 22.05.2014-24.05.2014 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova enzymatic activity ; inhibiting activity ; epididymal fluid ; proteinase Vědní obor RIV CE - Biochemie CEP P503/12/1834 GA ČR - Grantová agentura ČR P502/14/05547S GA ČR - Grantová agentura ČR CZ1.05/1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) Anotace Sperm maturation, represents a key step in the reproduction process. Spermatozoa, particularly the plasma membrane, are exposed to epididymal fluid (EF) components representing the natural environment essential for their post-testicular maturation. Changes in the sperm membrane proteins are influenced by proteolytic and glycosidic enzymes present in the EF. Accordingly, the occurrence of inhibitors in this reproductive organ is very important for the regulation of sperm membrane protein processing. In present study, we monitored protease and glycosidase activities, and inhibitors of metallo- and serine proteinases in boar EF. Additionally, we studied acrosin inhibitor in fluid, spermatozoa and tissue along the epididymis. We chromatographically separated boar EF into several fractions. These fractions were subjected to SDS-electrophoresis and the separated proteins were either studied by zymographic methods or transferred to nitrocellulose membranes for detection of metallo- and serine proteinases and their inhibitors, and acrosin inhibitor by specific antibody, respectively. Acrosin inhibitor was monitored also in the sperm and tissue of the boar epididymis. In boar epididymal fluid, several metallo- and serine proteinases with different molecular masses, and inhibitors of metalloproteinase MMP-9 and acrosin were found. We measured strong activity of mannosidase in this fluid. Using specific antibody, we registered the increasing signal of acrosin inhibitor from caput to cauda epididymis in the spermatozoa, fluid and also tissue. Proteinases and their inhibitors in reproductive fluids may play a significant role in reproduction processes. Especially, acrosin inhibitor in the reproductive tract inactivates prematurely released sperm acrosin and protects spermatozoa and reproductive epithelium against proteolytic degradation. High mannosidase activity in boar EF suggests evident role of mannose structures in the sperm interaction during reproductive events. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
Počet záznamů: 1