Počet záznamů: 1
Nonlethal screening of bat-wing skin with the use of ultraviolet fluorescence to detect lesions indicative of white-nose syndrome
- 1.
SYSNO ASEP 0428506 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nonlethal screening of bat-wing skin with the use of ultraviolet fluorescence to detect lesions indicative of white-nose syndrome Tvůrce(i) Turner, G. G. (US)
Meteyer, C. U. (US)
Barton, H. (US)
Gumbs, J. F. (US)
Reeder, D. M. (US)
Overton, B. (US)
Banďouchová, H. (CZ)
Bartonička, T. (CZ)
Martínková, Natália (UBO-W) RID, ORCID, SAI
Pikula, J. (CZ)
Zukal, Jan (UBO-W) RID, ORCID, SAI
Blehert, D. S. (US)Celkový počet autorů 12 Zdroj.dok. Journal of Wildlife Diseases - ISSN 0090-3558
Roč. 50, č. 3 (2014), s. 566-573Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova bats ; Chiroptera ; dermatomycosis ; fungal infection ; ultraviolet (UV) fluorescence ; white-nose syndrome Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEP GAP506/12/1064 GA ČR - Grantová agentura ČR Institucionální podpora UBO-W - RVO:68081766 UT WOS 000338909600017 EID SCOPUS 84901281196 DOI 10.7589/2014-03-058 Anotace Definitive diagnosis of the bat disease white-nose syndrome (WNS) requires histologic analysis to identify the cutaneous erosions caused by the fungal pathogen Pseudogymnoascus [formerly Geomyces] destructans (Pd). Gross visual inspection does not distinguish bats with or without WNS, and no nonlethal, on-site, preliminary screening methods are available for WNS in bats. We demonstrate that long-wave ultraviolet (UV) light (wavelength 368–385 nm) elicits a distinct orange–yellow fluorescence in bat-wing membranes (skin) that corresponds directly with the fungal cupping erosions in histologic sections of skin that are the current gold standard for diagnosis of WNS. Between March 2009 and April 2012, wing membranes from 168 North American bat carcasses submitted to the U.S. Geological Survey National Wildlife Health Center were examined with the use of both UV light and histology. Comparison of these techniques showed that 98.8% of the bats with foci of orange–yellow wing fluorescence (n580) were WNS-positive based on histologic diagnosis; bat wings that did not fluoresce under UV light (n588) were all histologically negative for WNS lesions. Punch biopsy samples as small as 3 mm taken from areas of wing with UV fluorescence were effective for identifying lesions diagnostic for WNS by histopathology. In a nonlethal biopsy-based study of 62 bats sampled (4-mm diameter) in hibernacula of the Czech Republic during 2012, 95.5% of fluorescent (n522) and 100% of nonfluorescent (n540) wing samples were confirmed by histopathology to be WNS positive and negative, respectively. This evidence supports use of longwave UV light as a nonlethal and field-applicable method to screen bats for lesions indicative of WNS. Further, UV fluorescence can be used to guide targeted, nonlethal biopsy sampling for follow-up molecular testing, fungal culture analysis, and histologic confirmation of WNS. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2015
Počet záznamů: 1