Počet záznamů: 1
Rapid enzymatic hydrolysis of masked deoxynivalenol and zearalenone prior to liquid chromatography mass spectrometry or immunoassay analysis
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SYSNO ASEP 0428444 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Rapid enzymatic hydrolysis of masked deoxynivalenol and zearalenone prior to liquid chromatography mass spectrometry or immunoassay analysis Tvůrce(i) Nielen, M. F. V. (NL)
Weijers, C. A. G. M. (NL)
Peters, J. (NL)
Weignerová, Lenka (MBU-M) RID
Zuilhof, H. (NL)
Franssen, M. C. R. (NL)Zdroj.dok. World Mycotoxin Journal. - : Wageningen Academic Publishers - ISSN 1875-0710
Roč. 7, č. 2 (2014), s. 107-113Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova masked mycotoxins ; enzymatic hydrolysis ; 1,3-beta-glucanase Vědní obor RIV CE - Biochemie Institucionální podpora MBU-M - RVO:61388971 UT WOS 000334568500001 DOI 10.3920/WMJ2013.1662 Anotace Recently it has been shown that conjugates ('masked mycotoxins') may contribute to the total daily intake of hazardous mycotoxins. Therefore, there is an urgent need for rapid analysis methods that assess the level of both free and masked mycotoxins in food and feed. However, the analysis of masked mycotoxins by either immunoassays or instrumental methods, such as liquid chromatography tandem mass spectrometry (LC-MS/MS), is severely hindered by the lack of standards and the unpredictable cross-reactivity profiles of the available antibodies. In this work, 26 enzymes were explored for rapid hydrolysis of masked mycotoxins using deoxynivaleno1-3-glucoside (DON-3G) as model compound. Following initial screening, the most promising enzyme, a fungal 1,3-beta-glucanase (laminarinase), was investigated in detail and found to be fit-for-purpose, providing complete conversions in minutes rather than hours according to LC-MS/MS analyses. As a proof of concept, the enzymatic pretreatment was applied to an extract of beer containing DON-3G. In addition, the feasibility of a fully automated enzymatic pretreatment of masked mycotoxin standards in an autosampler was demonstrated in an imaging surface plasmon resonance immunoassay set-up. Such an automated pretreatment was found to be equally applicable to other mycotoxin conjugates, as shown by the conversion of zearalenone-14-beta-D-glucopyranoside and zearalenone-14-sulphate, in the latter case using a sulphatase enzyme. It is envisaged that laminarinase could be useful for other masked mycotoxins as well Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1