Počet záznamů: 1
Selective chromogenic and fluorogenic peptide substrates for the assay of cysteine peptidases in complex mixtures
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SYSNO ASEP 0424813 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Selective chromogenic and fluorogenic peptide substrates for the assay of cysteine peptidases in complex mixtures Tvůrce(i) Semashko, T. A. (RU)
Vorotnikova, E. A. (RU)
Sharikova, V. F. (RU)
Vinokurov, Konstantin (BC-A) RID, ORCID
Smirnova, Y. A. (RU)
Dunaevsky, Y. E. (RU)
Belozersky, M. A. (RU)
Oppert, B. (US)
Elpidina, E. N. (RU)
Filippova, I. Y. (RU)Celkový počet autorů 10 Zdroj.dok. Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 449, č. 1 (2014), s. 179-187Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cysteine peptidases ; substrates of peptidases ; selective peptide substrates Vědní obor RIV CE - Biochemie Institucionální podpora BC-A - RVO:60077344 UT WOS 000332816100026 EID SCOPUS 84893337071 DOI 10.1016/j.ab.2013.12.032 Anotace This study describes the design, synthesis, and use of selective peptide substrates for cysteine peptidases of the C1 papain family, important in many biological processes. The structure of the newly synthesized substrates is Glp-Xaa-Ala-Y (where Glp = pyroglutamyl; Xaa = Phe or Val; and Y = pNA [p -nitroanilide], AMC [4-amino-7-methylcoumaride], or AFC [4-amino-7- trifluoromethyl-coumaride]). Substrates were synthesized enzymatically to guarantee selectivity of the reaction and optical purity of the target compounds, simplifying the scheme of synthesis and isolation of products. The hydrolysis of the synthesized substrates was evaluated by C1 cysteine peptidases from different organisms and with different functions, including plant enzymes papain, bromelain, ficin, and mammalian lysosomal cathepsins B and L. The new substrates were selective for C1 cysteine peptidases and were not hydrolyzed by serine, aspartic, or metallo peptidases. We demonstrated an application of the selectivity of the synthesized substrates. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2015 Elektronická adresa http://www.sciencedirect.com/science/article/pii/S0003269713006180#
Počet záznamů: 1