Počet záznamů: 1
Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus
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SYSNO ASEP 0422632 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus Tvůrce(i) De Winter, K. (BE)
Šimčíková, Daniela (MBU-M)
Schlack, B. (BE)
Weignerová, Lenka (MBU-M) RID
Pelantová, Helena (MBU-M) ORCID, RID
Soetaert, W. (BE)
Desmet, T. (BE)
Křen, Vladimír (MBU-M) RID, ORCIDZdroj.dok. Bioresource Technology. - : Elsevier - ISSN 0960-8524
Roč. 147, NOV 2013 (2013), s. 640-644Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova alpha-L-Rhamnosidase ; Aspergillus terreus ; Glycosylation Vědní obor RIV CE - Biochemie CEP LD13042 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 7E11011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000326660900088 DOI 10.1016/j.biortech.2013.08.083 Anotace This study describes an efficient, large scale fermentation of a recombinant alpha-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of alpha-L-rhamnosides among which cyclohexyl alpha-L-rhamnopyranoside, anisyl alpha-L-rhamnopyranoside and 2-phenylethyl alpha-L-rhamnopyranoside. In addition, alpha-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1