Počet záznamů: 1
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
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SYSNO ASEP 0422562 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study Tvůrce(i) Benkovská, Dagmar (UIACH-O)
Flodrová, Dana (UIACH-O)
Bobálová, Janette (UIACH-O) RID, ORCID
Laštovičková, Markéta (UIACH-O) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770 Rozsah stran s. 296-298 Poč.str. 3 s. Forma vydání Online - E Akce CECE Junior 2013 Datum konání 12.11.2013-13.11.2013 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova glycosylation ; post-translational modification ; barley ; N-glycopetptides Vědní obor RIV CB - Analytická chemie, separace CEP EE2.3.20.0182 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GPP503/12/P395 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000328730800005 Anotace N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2014
Počet záznamů: 1