Počet záznamů: 1
Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences
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SYSNO ASEP 0422254 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences Tvůrce(i) Matyášek, Roman (BFU-R) ORCID
Fulneček, Jaroslav (BFU-R)
Kovařík, Aleš (BFU-R) RID, ORCIDCelkový počet autorů 3 Zdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 34, č. 17 (2013), s. 2511-2521Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova HIGHLY REPETITIVE DNA ; DOUBLE-HELICAL DNA ; CURVED DNA Vědní obor RIV AC - Archeologie, antropologie, etnologie CEP GA206/09/1751 GA ČR - Grantová agentura ČR GAP501/10/0208 GA ČR - Grantová agentura ČR GA13-10057S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 CEZ AV0Z50040702 - BFU-R (2007-2013) UT WOS 000327667000007 EID SCOPUS 84883310801 DOI 10.1002/elps.201300227 Anotace DNA containing a sequence that generates a local curvature exhibits a pronounced retardation in electrophoretic mobility. Various theoretical models have been proposed to explain relationship between DNA structural features and migration anomaly. Here, we studied the capacity of 15 static wedge-bending models to predict electrophoretic behavior of 69 satellite monomers derived from four divergent families. All monomers exhibited retarded mobility in PAGE corresponding to retardation factors ranging 1.02-1.54. The curvature varied both within and across the groups and correlated with the number, position, and lengths of A-tracts. Two dinucleotide models provided strong correlation between gel mobility and curvature prediction; two trinucleotide models were satisfactory while remaining dinucleotide models provided intermediate results with reliable prediction for subsets of sequences only. In some cases, similarly shaped molecules exhibited relatively large differences in mobility and vice versa. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2014
Počet záznamů: 1