Počet záznamů: 1
Split Photosystem Protein, Linear-Mapping Topology, and Growth of Structural Complexity in the Plastid Genome of Chromera velia
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SYSNO ASEP 0422243 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Split Photosystem Protein, Linear-Mapping Topology, and Growth of Structural Complexity in the Plastid Genome of Chromera velia Tvůrce(i) Janouškovec, J. (CA)
Sobotka, Roman (MBU-M) RID, ORCID
Lai, De Hua (BC-A)
Flegontov, Pavel (BC-A) RID, ORCID
Koník, P. (CZ)
Komenda, Josef (MBU-M) RID, ORCID
Ali, S. (SA)
Prášil, Ondřej (MBU-M) RID, ORCID
Pain, A. (SA)
Oborník, Miroslav (MBU-M) RID
Lukeš, Julius (BC-A) RID, ORCID
Keeling, P. J. (CA)Zdroj.dok. Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038
Roč. 30, č. 11 (2013), s. 2447-2462Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova plastid genome evolution ; Chromera velia ; split protein Vědní obor RIV CE - Biochemie CEP GAP506/12/1522 GA ČR - Grantová agentura ČR GBP501/12/G055 GA ČR - Grantová agentura ČR ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000326745300006 DOI 10.1093/molbev/mst144 Anotace The canonical photosynthetic plastid genomes consist of a single circular-mapping chromosome that encodes a highly conserved protein core, involved in photosynthesis and ATP generation. Here, we demonstrate that the plastid genome of the photosynthetic relative of apicomplexans, Chromera velia, departs from this view in several unique ways. Core photosynthesis proteins PsaA and AtpB have been broken into two fragments, which we show are independently transcribed, oligoU-tailed, translated, and assembled into functional photosystem I and ATP synthase complexes. Genome-wide transcription profiles support expression of many other highly modified proteins, including several that contain extensions amounting to hundreds of amino acids in length. Canonical gene clusters and operons have been fragmented and reshuffled into novel putative transcriptional units. Massive genomic coverage by paired-end reads, coupled with pulsed-field gel electrophoresis and polymerase chain reaction, consistently indicate that the C. velia plastid genome is linear-mapping, a unique state among all plastids. Abundant intragenomic duplication probably mediated by recombination can explain protein splits, extensions, and genome linearization and is perhaps the key driving force behind the many features that defy the conventional ways of plastid genome architecture and function Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1