Počet záznamů: 1
In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil
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SYSNO ASEP 0421276 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil Tvůrce(i) Zweygarth, E. (DE)
Schol, H. (DE)
Lis, K. (DE)
Cabezas Cruz, Alejandro (BC-A)
Thiel, C. (DE)
Silaghi, C. (DE)
Ribeiro, M.F.B. (BR)
Passos, L.M.F. (BR)Zdroj.dok. Transboundary and Emerging Diseases. - : Wiley - ISSN 1865-1674
Roč. 60, NOV 2013 (2013), s. 86-92Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Ehrlichia ; Rhipicephalus (Boophilus) microplus ; in vitro culture ; tick cell ; DH82 ; endothelial cell ; cattle ; 16S rRNA ; Brazil Vědní obor RIV EB - Genetika a molekulární biologie Institucionální podpora BC-A - RVO:60077344 UT WOS 000326839500013 DOI 10.1111/tbed.12122 Anotace Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3months, corresponding to six subcultures, whereas the other two infected cell lines were kept for more than 1year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells with organisms grown in DH82 cells was achieved. Sequence analysis made with a fragment of the 16S rRNA gene showed that this Ehrlicha sp. is closely related to Ehrlichia canis. However, the maximum likelihood phylogenetic tree shows that it falls in a separate phylogenetic clade from E.canis. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2014
Počet záznamů: 1