Počet záznamů: 1  

Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies

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    SYSNO ASEP0421260
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevHumoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies
    Tvůrce(i) Wagnerová, Pavla (BC-A)
    Sak, Bohumil (BC-A) RID
    Květoňová, Dana (BC-A)
    Maršálek, M. (CZ)
    Langerová, I. (CZ)
    Kváč, Martin (BC-A) RID
    Zdroj.dok.Veterinary Parasitology. - : Elsevier - ISSN 0304-4017
    Roč. 197, 1-2 (2013), s. 1-6
    Poč.str.6 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaPonies ; Encephalitozoon cuniculi genotype II ; PCR ; Antibodies ; Experimental infection
    Vědní obor RIVEC - Imunologie
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000324787400001
    AnotaceA total of 9 (8 stallions and 1 mare) 1 year old ponies were used for the experimental infection caused by Encephalitozoon cuniculi genotype II (107 spores per animal). Subsequently, individual horses were slaughtered 7, 14, 21, 28, 35, 42, 49, 56, and 63 days post infection. Immediately after slaughter, tissues samples of stomach, duodenum, jejunum, ileum, caecum, colon, spleen, liver, kidney, bladder, heart, lungs, and brain were sampled. In addition, urine, feces and blood specimens were collected. Enzyme-linked immunosorbent assay was used for determination of humoral immune response and nested PCR targeting 16S rDNA, whole ITS, and 5.8S rDNA was used for detection of E. cuniculi in collected organs, blood, feces and urine. No clinical signs of microsporidiosis including diarrhea or colic, neurological signs and fever were observed in any horses during whole experiment. Acute microsporidiosis in ponies was characterized by the dissemination of microsporidia into almost all organs and significant increase of concentration of specific antibodies in blood was observed from 28 to 42 DPI. After this acute stage microsporidia disappeared from most organs with the exception of the kidney, which was positive up to 63 DPI when the experiment was terminated. No pathological changes were observed in any organs with exception of one mare's brain, where E. cuniculi-positive cavity measuring 5cm x 3 cm in diameter formed in the lobus pinformis.
    PracovištěBiologické centrum (od r. 2006)
    KontaktVáclava Lavičková, lavickova@hbu.cas.cz, Tel.: 387 775 889
    Rok sběru2014