Počet záznamů: 1
Characterization of Two Mitochondrial Flavin Adenine Dinucleotide-Dependent Glycerol-3-Phosphate Dehydrogenases in Trypanosoma brucei
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SYSNO ASEP 0421249 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of Two Mitochondrial Flavin Adenine Dinucleotide-Dependent Glycerol-3-Phosphate Dehydrogenases in Trypanosoma brucei Tvůrce(i) Škodová, Ingrid (BC-A) ORCID
Verner, Zdeněk (BC-A)
Bringaud, F. (FR)
Fabian, P. (SK)
Lukeš, Julius (BC-A) RID, ORCID
Horváth, A. (SK)Zdroj.dok. Eukaryotic Cell - ISSN 1535-9778
Roč. 12, č. 12 (2013), s. 1664-1673Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova alternative NADH dehydrogenase ; inducible expression system ; blood-stream forms ; complex-I ; procyclic trypanosomes ; sleeping sickness ; oxidase ; localization ; metabolism ; cycle Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/11/2179 GA ČR - Grantová agentura ČR GD206/09/H026 GA ČR - Grantová agentura ČR LH12104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 UT WOS 000327712200010 DOI 10.1128/EC.00152-13 Anotace Glycerol-3-phosphate dehydrogenases (G3PDHs) constitute a shuttle that serves for regeneration of NAD(+) reduced during glycolysis. This NAD-dependent enzyme is employed in glycolysis and produces glycerol-3-phosphate from dihydroxyacetone phosphate, while its flavin adenine dinucleotide (FAD)-dependent homologue catalyzes a reverse reaction coupled to the respiratory chain. Trypanosoma brucei possesses two FAD-dependent G3PDHs. While one of them (mitochondrial G3PDH [mtG3PDH]) has been attributed to the mitochondrion and seems to be directly involved in G3PDH shuttle reactions, the function of the other enzyme (putative G3PDH [putG3PDH]) remains unknown. In this work, we used RNA interference and protein overexpression and tagging to shed light on the relative contributions of both FAD-G3PDHs to overall cellular metabolism. Our results indicate that mtG3PDH is essential for the bloodstream stage of T. brucei, while in the procyclic stage the enzyme is dispensable. Expressed putG3PDH-V5 was localized to the mitochondrion, and the data obtained by digitonin permeabilization, Western blot analysis, and immunofluorescence indicate that putG3PDH is located within the mitochondrion. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2014
Počet záznamů: 1