Počet záznamů: 1

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

SYSNO ASEP	0420971
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe
Tvůrce(i)	Němejc, K. (CZ) Sak, Bohumil (BC-A)_{RID, ORCID} Květoňová, Dana (BC-A)_SAI Hanzal, V. (CZ) Janiszewski, P. (PL) Forejtek, P. (CZ) Rajský, D. (SK) Ravaszová, P. (SK) McEvoy, J. (US) Kváč, Martin (BC-A)_{RID, RID, ORCID}
Zdroj.dok.	Veterinary Parasitology. - : Elsevier - ISSN 0304-4017 Roč. 197, 3-4 (2013), s. 504-508
Poč.str.	5 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Central Europe ; Cryptosporidium scrofarum ; Cryptosporidium suis ; Eurasian wild boar ; PCR ; SSU
Vědní obor RIV	EE - Mikrobiologie, virologie
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000328010500012
DOI	10.1016/j.vetpar.2013.07.003
Anotace	From 2011 to 2012, to identify Cryptosporidium spp. occurrence in Eurasian wild boars (Sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the Central European countries of Austria, the Czech Republic, Poland, and the Slovak Republic were investigated. Cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. Sixty-one Cryptosporidium infections, including the 11 infections that were detected by microscopy, were detected using genus- or species-specific nested PCR amplification of SSU rDNA. This represents a 5.5 fold greater sensitivity for PCR relative to microscopy. Combining genus- and species-specific PCR tools significantly changes the perspective on the occurrence of Cryptosporidium spp. in wild boars. While RFLP and direct sequencing of genus specific PCR-amplified products revealed 56 C. suis (20) and C. scrofarum (36) monoinfections and only 5 mixed infections of these species, species-specific molecular tools showed 44 monoinfections and 17 mixed infections with these species. PCR analysis of the gp60 gene did not reveal any other Cryptosporidium infections. Similar to domestic pigs, C. scrofarum was detected as a dominant species infecting adult Eurasian wild boars (Sus scrofa). Cryptosporidium infected wild boars did not show signs of clinical disease. This report is perhaps the most comprehensive survey of cryptosporidial infection in wild boars.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2014

Počet záznamů: 1